Meat Allergy : Investigation of Potential Allergenic Proteins in Beef
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概要
- 論文の詳細を見る
The potential allergenic proteins in beef were investigated. The sera of ten beef-allergic patients suffering from atopic dermatitis and having a positive RAST score to beef, aged 3-18 years, were obtained from Yoshida Hospital in Japan, and five non-allergic individuals were subjected to this study. The sera of the ten patients reacted strongly to a beef extract, but not to pork and chicken extracts by both ELISA and immunoblotting. The sera of the five control subjects did not react to any of these meat extracts. Three bands having molecular masses of 〜200 kDa, 〜67 kDa and 〜60 kDa were observed by immunoblotting after SDS-PAGE. Two fractions of the beef extract from a Sephadex-gel (G-200) filtration column strongly reacted with the sera of the beef-allergic patients by ELISA and immunoblotting : one fraction had the 〜67 kDa component and the other had the 〜200 kDa and 〜60 kDa components. One of them (〜67 kDa) was confirmed to be bovine serum albumin (BSA) by an analysis of the N-terminal amino acid sequence. We could not identify the others by sequencing, but the 〜200 kDa and 〜60 kDa components were presumed to be glycoproteins. Bovine gamma globulin (BGG : M.W. 〜160 kDa) is a glycoprotein and has several subunits. The beef-allergic patients showed strong reactivity to the 〜200 kDa and 〜60 kDa components of pure BGG by immunoblotting. Inhibition-ELISA showed that pure BGG preparations strongly inhibited the binding of sera from the beef-allergic patients to the beef extract. These results suggest that the 〜200 kDa, 〜67 kDa and 〜60 kDa components in the beef extract had strong allergenicity : 〜67 kDa was BSA, and 〜200 kDa and 〜60 kDa were presumably aggregated BGG and it's heavy chain, respectively.
- 社団法人日本農芸化学会の論文
- 2000-09-23
著者
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Suzuki Atsushi
Department of Cardiology, Musashino Red Cross Hospital
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IKEUCHI Yoshihide
Department of Animal Science, Faculty of Agriculture, University of Niigata
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Suzuki Atsushi
Biodevelopment Division Central Institute Nagoya Seiraku Co. Ltd.
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Suzuki Atsushi
Department Of Applied Biological Chemistry Faculty Of Agriculture University Of Niigata
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Suzuki Atsushi
Department Of Animal Husbandry Faculty Of Agriculture Tohoku University
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Suzuki Atsushi
Synthetic Crystal Research Laboratory Faculty Of Engineering Nagoya University
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Suzuki Atsushi
Department Of Pathology Obstetrics And Gynecology Keio University School Of Medicine
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Suzuki Atsushi
Basic Research Department Prima Meat Packers Co. Ltd.
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Suzuki Atsushi
Department Of Applied Biochemistry Faculty Of Agriculture University Of Niigata
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Suzuki A
Department Of Obstetrics And Gynecology Keio University School Of Medicine
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Suzuki A
Department Of Applied Biological Chemistry Faculty Of Agriculture University Of Niigata
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HAN Gi
Doctor's Program in Functional Biology, Graduate School of Science and Technology, University of Nii
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MATSUNO Masatomo
Department of Pediatrics, Yoshida Hospital
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IKEUCHI Yoshihide
Division of Food Animal Science, Graduate School of Agricultural Sciences, Kyushu University
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HAN GiDong
Doctor's Program in Functional Biology, Graduate School of Science and Technology, University of Nii
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ITO Giichi
Health Administration Center, University of Niigata
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Han Gidong
Doctor's Program In Functional Biology Graduate School Of Science And Technology University Of
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Ito Giichi
Health Administration Center University Of Niigata
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Ikeuchi Y
Division Of Food Animal Science Graduate School Of Agricultural Sciences Kyushu University
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Matsuno Masatomo
Department Of Pediatrics Yoshida Hospital
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Ikeuchi Yoshihide
Department Of Animal Science Faculty Of Agriculture University Of Niigata
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