Prothoracicotropic Hormone of the Silkworm, Bombyx mori : Amino Acid Sequence and Dimeric Structure(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Prothoracicotropic hormone (PTTH) of the silkworm, Bombyx mori, was purified and its primary structure determined for the most part. From sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified PTTH under non-reducing and reducing conditions, and the amino acid sequence and composition analyses of the carboxamidomethylated PTTH, we concluded that Bombyx PTTH has a dimeric structure consisting of two identical, or nearly identical subunits, held together by disulfide bond(s). There exist subunit variants that differ by deletion of only a few amino acid residues at the N-terminus, and possibly at the C-terminus also, giving rise to a high heterogeneity in the PTTH molecule. The amino acid sequence up to the 104th residue from the N-terminus of the longest subunit, except for the 41st residue, was determined by sequence analysis of fragment peptides produced by lysyl endopeptidase, chymotrypsin and V8 protease digestions, leaving only a short C-terminal sequence undetermined. Bombyx PTTH is expected to contain a carbohydrate chain bound to an asparagine at position 41, deduced from the cDNA sequence.
- 社団法人日本農芸化学会の論文
- 1991-01-23
著者
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Suzuki A
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Suzuki A
Department Of Agricultural Chemistry The Uniersity Of Tokyo
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Suzuki Akinori
Department Of Agricultural Chemistry The University Of Tokyo
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Nagasawa Hiromichi
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Kataoka Hiroshi
Department of Applied Chemistry and Biotechnology, Niigata Institute of Technology
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Kataoka H
Univ. Tokyo Tokyo Jpn
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ISOGAI Akira
Department of Agricutural Chemistry, The University of Tokyo
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Kodama Hisashi
Central Research Institute Japan Tabacco Inc.
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ISHIZAKI Hironori
Biological Institute, Faculty of Science, Nagoya University
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Kataoka Hiroshi
Institute Of Applied Biochemistry University Of Tsukada
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Suzuki Akinori
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Nagasawa Hiromichi
Department Of Applied Biological Chemistry Graduate School Of Agriculture And Life Sciences Universi
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Nagasawa Hiromichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Nagasawa Hiromichi
Department Of Applied Biological Chmistry Graduate School Of Agricultural And Life Sciences The Univ
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Isogai Akira
Department Of Agricultural Chemistry The University Of Tokyo
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石崎 宏矩
Nagoya Univ.
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Suzuki Akinori
Chemistry Faculty Of Agriculture The University Of Tokyo
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Isogai Akira
Department Of Applied Biological Chemistry The University Of Tokyo
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Isogai Akira
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Kataoka Hiroshi
Department Of Applied Biological Technology The University Of Tokyo
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Suzuki A
Faculty Of Agriculture The University Of Tokyo
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KATAOKA Hiroshi
Department of Agricultural Chemistry, The University of Tokyo
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