Ultrastructure of Papillar Cells in Brassica campestris Revealed by Liquid Helium Rapid-Freezing and Substitution-Fixation Method

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概要

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• The ultrastructure of papillar cells of Brassica campestris on the day of anthesis was studied by the liquid helium rapid-freezing and a substitution-fixation method, abbreviated as the RFS method. Application of the RFS method to the analysis of papillar cells enabled us to examine clear images of these cells which have not been observed previously. The well-developed rough and smooth endoplasmic reticulum, numerous Golgi bodies and mitochondria, various small vesicles and clathrin-coated vesicles, were present in the cells. The numbers of Golgi bodies, as well as the numbers of cisternae of each Golgi body, increased as compared to that in the other cells of style. Lattice-like fenestrated and flattened cisternae were seen adjacent to the narrowest trans cisterna of the Golgi body, which had a partially coated region at its periphery. Many coated vesicles were observed in the vicinity of this structure and the plasma membrane. Coated areas on the plasma membrane were also observed. The ultrastructure of papillar cells on the day of anthesis indicated that they are very active secretory cells. By using an antibody against S_8-protein and sections prepared by the RFS method, we demonstrated the distribution of S_8-protein in the cell wall of papillar cells of homozygous plants of Brassica campestris S_8S_8.

• 日本植物生理学会の論文

著者

• Watanabe M

  Lab. Of Plant Reproductive Genetics Graduate School Of Life Sciences Tohoku Univ.

• Suzuki A

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Suzuki Akinori

  Department Of Agricultural Chemistry The University Of Tokyo

• Watanabe Masao

  Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University

• ISOGAI Akira

  Department of Agricutural Chemistry, The University of Tokyo

• Kishi-nishizawa Naoko

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Hinata Kokichi

  Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University

• Yamakawa Seiei

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

• Shojima Shinsuke

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

• Yamakawa Seiyei

  Graduate School Of Biological Science Nara Institute Of Science And Technology

• Yamakawa Seiyei

  Technology Department Research Association For Biotechnology

• Sakagami Y

  Nagoya Univ.

• Suzuki Akinori

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Hinata Kokichi

  Laboratory Of Plant Breeding Faculty Of Agriculture Tohoku University

• Yamakawa Seiyei

  School Of Agricultural Sciences Nagoya University

• Suzuki Akinori

  Chemistry Faculty Of Agriculture The University Of Tokyo

• Shojima Shinsuke

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Isogai Akira

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Watanabe Masao

  Laboratory Of Plant Reproductive Genetics Graduate School Of Life Sciences Tohoku University

• Suzuki A

  Faculty Of Agriculture The University Of Tokyo

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