Exploring Amino Acids Responsible for the Temperature Profile of Glycoside Hydrolase Family 45 Endoglucanase EGL3 from Humicola grisea
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概要
- 論文の詳細を見る
EGL3 and RCE1 are glycoside hydrolase family 45 endoglucanases isolated from Humicola grisea and Rhizopus oryzae respectively. The amino acid sequences of the two endoglucanases are homologous; on the other hand, the optimum temperature of EGL3 is higher than that of RCE1. In this study, four chimeric endoglucanases, named ER1, ER2, ER3 and ER4, in which one of four sequential amino acid regions of the EGL3 catalytic domain (CAD) was replaced by the corresponding RCE1 amino acids, were constructed to explore the region responsible for the EGL3 temperature profile. Then their temperature profiles were compared with that of the recombinant EGL3. Replacement of the N-terminal region of EGL3 with that of RCE1 caused the EGL3 temperature profile to shift to a lower temperature. These results suggest that the N-terminal amino acids of the EGL3 are responsible for the EGL3 temperature profile.
- 2007-09-23
著者
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Iwasaki Yusaku
School of Food and Nutritional Sciences, University of Shizuoka
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WATANABE Tatsuo
School of Food and Nutritional Sciences, University of Shizuoka
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KOBATA Kenji
School of Food and Nutritional Sciences, University of Shizuoka
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Watanabe Tatsuo
School Of Food And Nutritional Sciences Univ. Of Shizuoka
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MORITA Akihito
School of Food and Nutritional Sciences, University of Shizuoka
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YOKOGOSHI Hidehiko
School of Food and Nutritional Sciences and COE Program in the 21st Century, University of Shizuoka
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Yokogoshi Hidehiko
School Of Food And Nutritional Sciences And Coe Program In The 21st Century The University Of Shizuo
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Kono Toshiaki
Food And Health R&d Laboratories Meiji Seika Kaisha Ltd.
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Kobata Kenji
School Of Food And Nutritional Sciences And Coe Program In The 21st Century University Of Shizuoka
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Yokogoshi Hidehiko
School Of Food And Nutritional Sciences And Coe Program In The 21st Century University Of Shizuoka
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Iwasaki Yusaku
School Of Food And Nutritional Sciences And Coe Program In The 21st Century University Of Shizuoka
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Morita Akihito
School Of Food And Nutritional Sciences And Coe Program In The 21st Century University Of Shizuoka
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Kubota Hidetoshi
Food And Health R&d Laboratories Meiji Seika Kaisha Ltd.
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Watanabe Tatsuo
School Of Food And Nutritional Sciences And Coe Program In The 21st Century University Of Shizuoka
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Murashima Koichiro
School of Food and Nutritional Sciences and COE Program in the 21st Century, University of Shizuoka
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Shimonaka Atsushi
School of Food and Nutritional Sciences and COE Program in the 21st Century, University of Shizuoka
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Nishimura Tomoko
School of Food and Nutritional Sciences and COE Program in the 21st Century, University of Shizuoka
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