An Extracellular Quinoprotein Oxidase That Catalyzes Conversion of Enacyloxin IVa to Enacyloxin IIa
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概要
- 論文の詳細を見る
A new extracellular quinoprotein oxidase named enacyloxin oxidase (ENX oxidase), which is involved in biosynthesis of ENX IIa, a congener of ENX, was found in the culture supernatant of Frateuria sp. W-315 and purified as a homogeneous protein on SDS-PAGE. ENX oxidase was shown to have a molecular mass of 73kDa by SDS-PAGE and 79kDa by gel filtration. The enzyme was inhibited by various carbonyl reagents and the activity was stimulated by addition of PQQ. This is the first report on a quinoprotein oxidase that is secreted into the culture medium in the logarithmic growth phase, and acts for biosynthesis of the antibiotic.
- 社団法人日本農芸化学会の論文
- 1994-10-23
著者
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WATANABE Tatsuo
School of Food and Nutritional Sciences, University of Shizuoka
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Oyama Ryo
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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IZAKI Kazuo
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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WATANABE TOSHIHIKO
Department of Surgery, Tachikawa Kyosai Hospital
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SUGIYAMA Takeyoshi
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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Watanabe Tatsuo
Graduate School Of Nutritional And Environmental Sciences And Coe Program In The 21st Century Univ.
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Watanabe T
Department Of Bioscience And Biotechnology Graduate School Of Agriculture Kinki University
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Watanabe Tetsuro
Department Of Bioresources Chemistry Chiba University
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杉山 長美
Tohoku Univ. Sendai Jpn
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Izaki K
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Izaki Kazuo
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Toki S
Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe University
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Hanzawa Hiroko
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University
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Sano Taketo
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University
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Sano Taketo
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Sugiyama Takeyoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Hanzawa Hiroko
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Watanabe Toshihiko
Department Of Pediatric Surgery Saitama City Hospital
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Watanabe Toshihiko
Department Of Aeronautics And Astronautics University Of Tokyo
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