Purification and Properties of Acid Phosphatases from Axes and Cotyledons of Germinating Soybeans(Biological Chemistry)
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概要
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Acid phosphatases (EC 3.1.3.2) from the axis and cotyledon of germinating soybeans (Glycine max L.) were purified approximately 700-fold and 2100-fold, respectively, to electrophoretic homogeneity by ammonium sulfate fractionation, cation-exchange, concanavalin A-Sepharose 4B affinity, and hydroxyapatite chromatographies. This report describes the purification and characterization of the enzymes from both axis and cotyledon. Acid phosphatases from both organs had the following similar properties, although they have some differences in substrate specificity. (i) The enzyme was a glycoprotein. (ii) The native enzyme of approximate molecular weight of 100,000 consisted of two subunits, each with an apparent molecular weight of 50,000. (iii) The optimal pH was approximately 5.7-5.8, and the enzyme was stable at the pH range of 5.3-8.0. (iv) The apparent Km value with p-nitrophenyl phosphate as a substrate was 3.7-4.0×10^<-4>M. (v) The enzyme activity was inhibited by Cu^<2+>, Zn^<2+>, Pb^<2+>, Mo_7O_<24>^<6->, F^-, and PO_4^<3-> ions. (vi) The enzyme hydrolyzed various phosphorylated compounds non-specifically. (vii) The antiserum against the enzyme from axis crossreacted to that from cotyledon.
- 社団法人日本農芸化学会の論文
- 1990-03-23
著者
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金子 淳
東北大学大学院農学研究科応用微生物学分野
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Kaneko Jun
Tohoku Univ. Sendai Jpn
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IZAKI Kazuo
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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金子 淳
東北大農化
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Kaneko Jun
Department Of Applied Microbiology Graduate School Of Agricultural Science Tohoku University
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KAMIO Yoshiyuki
Department of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University
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OKUDA Shin-ichi
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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KUROIWA Mayumi
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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AOKI Kazuhiko
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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Izaki K
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Izaki Kazuo
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Aoki Kazuhiko
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Kuroiwa Mayumi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Kamio Yoshiyuki
Department Of Agricultural Chemistry Tohoku University
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Ito K
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Okuda Shin-ichi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University:(present Office)hachin
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Okuda Shin-ichi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Okuda Shin-ichi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University:(present Office)hachin
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