Occurrence of Agmatine Pathway for Putrescine Synthesis in Selenomonas ruminatium
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概要
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Selenomonas ruminantium synthesizes cadaverine and putrescine from L-lysine and L-ornithine as the essential constituents of its peptidoglycan by a constitutive lysine/ornithine decarboxylase (LDC/ODC). S. ruminantium grew normally in the presence of the specific inhibitor for LDC/ODC, DL-α-difluoromethylornithine, when arginine was supplied in the medium. In this study, we discovered the presence of arginine decarboxylase (ADC), the key enzyme in agmatine pathway for putrescine synthesis, in S. ruminantium. We purified and characterized ADC and cloned its gene (adc) from S. ruminantium chromosomal DNA. ADC showed more than 60% identity with those of LDC/ODC/ADCs from Gram-positive bacteria, but no similarity to that from Gram-negative bacteria. In this study, we also cloned the aguA and aguB genes, encoding agmatine deiminase (AguA) and N-carbamoyl-putrescine amidohydrolase (AguB), both of which are involved in conversion from agmatine into putrescine. AguA and AguB were expressed in S. ruminantium. Hence, we concluded that S. ruminantium has both ornithine and agmatine pathways for the synthesis of putrescine.
- 社団法人 日本農芸化学会の論文
著者
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ABE Naoki
Laboratory of Applied Microbiology, School of Food and Nutritional Sciences, University of Shizuoka
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KANEKO Jun
Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Division of Bioscience an
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KAMIO Yoshiyuki
Department of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University
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YAMAGUCHI Yoshihiro
Laboratory of Pharmaceutical Physical Chemistry, Faculty of Pharmaceutical Sciences, Kumamoto Univer
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LIAO Shaofu
Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricu
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POONPAIROJ Phuntip
Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricu
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KO Kyong-Cheol
Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricu
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Ko Kyong-cheol
Laboratory Of Applied Microbiology Department Of Microbial Biotechnology Graduate School Of Agricult
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TAKATUSKA Yumiko
Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricu
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