An N-terminal Region of LukF of Staphylococcal Leukocidin/γ-Hemolysin Crucial for the Biological Activity of the Toxin

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概要

• 論文の詳細を見る

• The two staphylococcal bi-component toxins, leukocidin and γ-hemolysin share LukF [Kamio et al, FEBS Lett., 321,15-18 (1993)]. This report identifies the pivotal amino acid residues in the N-terminal region of LukF for the leukocytolytic and hemolytic activities in the presence of LukS and Hlg2,respectively, measuring the toxin activiy of a series of LukF mutants with truncated N-terminals. The data obtained showed that the LukF mutant TF21,lacking 20 amino acid residues at the N-terminus of LukF, failed to have any hemolytic activity and had less 10% leukocytolytic activity than that of the intact LukF, while 16-residue truncations retained both toxin activities without loss. The LukF mutants lacking 18-through 19-residue segments from the N-terminus showed low toxin activity on both target cells. All mutants having no toxin activity were also not capable of binding to the human erythrocytes. It can thus be concluded that the 3-residue segment, L^<18>Y^<19>K^<20> of LukF is crucial for the biological activity of the toxin.

• 社団法人日本農芸化学会の論文
• 1998-07-23

著者

• Kawai Yasushi

  東北大学 農学研究科

• 金子 淳

  東北大学大学院農学研究科応用微生物学分野

• Kaneko Jun

  Tohoku Univ. Sendai Jpn

• Huda Md.

  Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University

• Huda Md.nazmul

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• 金子 淳

  東北大農化

• Kaneko Jun

  Laboratory Of Applied Microbiology Department Of Microbial Biotechnology Graduate School Of Agricult

• Kaneko Jun

  Department Of Applied Microbiology Graduate School Of Agricultural Science Tohoku University

• TOMITA Toshio

  Department of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University

• KAMIO Yoshiyuki

  Department of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University

• MASCARENAS Ma.AnitaL.

  Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University

• Mascarenas Ma.anital.

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• Tomita T

  Showa Sangyo Co. Ltd.

• Tomita Toshio

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• Kamio Yoshiyuki

  Department Of Agricultural Chemistry Tohoku University

• Mascarenas Ma.Anita

  Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University

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