A Simple Purification Method and Morphology and Component Analyses for Carotovoricin Er, a Phage-tail-like Bacteriocin from the Plant Pathogen Erwinia carotovora Er
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概要
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Carotovoricin Er has been isolated as a phage-tai-like bacteriocin from the plant pathogen Erwinia carotovora Er [Kamimiya, S.et al., (1977), Agric.Biol.Chem.41,911-912]. However, the fine morphology and structural composition of carotovoricin Er remained to be studied because a large amount of contracted carotovoricin Er were present in the bacteriocin preparations so far obtained. To obtain intact carotovoricin Er and its major parts, we developed simple and efficient purification methods including the use of sucrose density gradient centrifugation in the presence of 10-20% (v/v) ethanol. Electron microscopy for the purified carotovoricin Er showed the presence of a novel antenna-like structure at the proximal end of the phage-tail-like particle, which consisted of a sheath-and-core part, a baseplate, and tail fibers. Contracted sheath and inner core were purified as hollow cylindrical structures with longitudinal lengths of 69 and 174nm, respectively, and tail fibers were purified as a fibrous structure with length of 63nm. SDS-polyacrylamide gel electrophoresis showed the presence of single major proteins of 50,20,and 68kDa in the isolated sheath, core, and tail fiber, respectively. Three other minor proteins of 46,44,and 35kDa were also identified as the structural proteins of carotovoricin Er, which may be the candidate proteins for the antenna-like and the base plate structures. Thus carotovoricin Er consists of at least 6 protein components.
- 社団法人日本農芸化学会の論文
- 1999-08-23
著者
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Kamio Yoshiyuki
Laboratory Of Applied Microbiology Department Of Molecular And Cell Biology Graduate School Of Agric
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Kawai Yasushi
東北大学 農学研究科
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Sato Takahide
Department Of Bioproduction Science Faculty Of Horticulture Chiba University
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Nguyen Anh
Laboratory Of Applied Microbiology Tohoku University
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Sato Takako
The Deepstar Group Japan Marine Science And Technology Center
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Sato T
Institute Of Applied Biochemistry University Of Tsukuba
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TOMITA Toshio
Laboratory of the Applied Microbiology, Department of Molecular and Cell Biology, Graduate School of
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HIROTA Morihiko
Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricu
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SATO Toshitsugu
Iwate Biotechnology Research Center
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SATO Tsuruji
Division of Electron Microscopy, Graduate School of Agricultural Science, Tohoku University
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Tomita T
Showa Sangyo Co. Ltd.
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Kamio Yoshiyuki
Laboratory Of Applied Microbiology Department Of Microbial Biotechnology Graduate School Of Agricult
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Sato T
Iwate Biotechnology Research Center:department Of Biotechnology And Environmental Chemistry National
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Hirota Morihiko
Laboratory Of Applied Microbiology Department Of Microbial Biotechnology Graduate School Of Agricult
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Hirota Morihiko
Laboratory Of Applied Microbiology Tohoku University
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Sato Tsutomu
Graduate School Of Science And Technology Faculty Of Agriculture Niigata University
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