Primary Amino Acid and DNA Sequences of Gassericin T, a Lactacin F-Family Bacteriocin Produced by Lactobacillus gasseri SBT2055
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概要
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A broad-spectral bacteriocin, named gassericin T, produced by Lactobacillus gasseri SBT 2055 (from human feces) was isolated to homogeneity from the culture supernatant by hydrophobic chromatography. By SDSPAGE and in situ activity assay, the purified gassericin T migrated as a single band with bacteriocin activity and molecular size of 5,400. A 2.9-kbp HindIII-HindIII fragment of chromosome DNA was hybridized with the oligonucleotide probe designed from the partial N-terminal amino acid sequence of gassericin T and was cloned. Six ORFs including the structural gene of gassericin T were deduced by computer analysis and the data bases. The structural gene of gassericin T (gatA) was identified as the fourth ORF, which encoded a protein composed of 75 amino acids that included the GG motif of the cleavage site. Chemical sequencing analysis of the complete amino acid sequence showed that gassericin T (57 amino acids) had a disulfide bond in the molecule and no modified amino acid residues, making it a class II bacteriocin. The gassericin T had 60% sequence similarity to mature LafA (57 amino acids, lactacin F, bacteriocins produced by L. johnsonii VPI11088), and the sequences around the processing site and C-terminal area were well conserved. The fifth ORF was designated as gatX, encoded as a peptide composed of 65 amino acids containing the GG motif of the putative cleavage site, however mature GatX and its antibacterial activity were not detected in the culture supernatant. GatX has higher similarity with LafX than with lactobin A (50 amino acids) belonging to the first lactacin F-family. These results indicated that gassericin T belongs to the hydrophobic class II bacteriocins and the most vicinal lactacin F-family.
- 社団法人日本農芸化学会の論文
- 2000-10-23
著者
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Kawai Yasushi
東北大学 農学研究科
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Kawai Yoshiyuki
Tokyo Dietitian Academy
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KITAZAWA Haruki
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
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SAITO Tadao
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
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Kawai Y
Kyoto Univ. Kyoto
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Itoh Tomoyuki
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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KAWAI Yasushi
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
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SAITOH Boku
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
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ITOH Takatoshi
Laboratory of Animal Food Science, College of Bioresource Science, Nihon University
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Saitoh Boku
Laboratory Of Animal Products Chemistry Graduate School Of Agricultural Science Tohoku University
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Itoh T
Laboratory Of Animal Food Science College Of Bioresource Science Nihon University
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Kawai Y
Faculty Of Veterinary And Animal Science Nippon Veterinary And Animal Science University
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Itoh T
Faculty Of Agriculture Iwate University
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Itoh T
Hiroshima Univ. Higashi-hiroshima Jpn
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TAKAHASHI Osamu
Laboratory of Animal Products Chemistry, Biological Resource Science, Graduate School of Agricultura
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NAKAJIMA Hadjime
Technology and Research Institute, Snow Brand Milk Products Co. Ltd.
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Kitazawa Haruki
Laboratory Of Animal Products Chemistry Graduate School Of Agricultural Science Tohoku University
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神尾 好是
東北大学
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Nakajima Hadjime
Technology And Research Institute Snow Brand Milk Products Co. Ltd.
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Takahashi Osamu
Laboratory Of Animal Products Chemistry Biological Resource Science Graduate School Of Agricultural
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北澤 春樹
国立大学法人東北大学大学院農学研究科
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Saito Tadao
Laboratory Of Animal Products Chemistry Graduate School Of Agricultural Science Tohoku University
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Takahashi Osamu
Laboratory for Brainware System, Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, Sendai 980-8577 Japan
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