Purification and Characterization of D-Xylose Isomerase from Bifidobacterium adolescentis

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概要

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• D-Xylose isomerase was purified to homogeneity from cell-free extracts of Bifidobacterium adolescentis by ammonium sulfate fractionation and chromatographies on DEAE-cellulose and Butyl-Toyopearl. The molecular weight of the purified enzyme was estimated to be 168,000 by gel filtration on TSKgel G-3000SW, and 53,000 on SDS-polyacrylamide gel electrophoresis. The optimum pH was around 7 and the enzyme was stable at pH 7-8. The enzyme required bivalent cations, Mg^<2+>, Co^<2+>, or Mn^<2+> for the activity, particularly Mn^<2+> to be best. The enzyme had a pI of 4.3, and the K_m for D-xylose was 4mM. The N-terminal amino acid sequence of the enzyme was not similar to those of D-xylose isomerases from other sources such as Clostridium thermosulfurogenes, Escherichia coli, or Bacillus subtilis.

• 社団法人日本農芸化学会の論文
• 1994-04-23

著者

• Sakurai Hiroaki

  Research Center, Nippon Beet Sugar Mfg. Co., Ltd.

• Yasushi Kawai

  Laboratory Of Animal Products Chemistry Graduate School Of Agricultural Science Tohoku University

• Sakurai Hidetoshi

  Department Of Chemistry And Life Science Nihon University

• SUZUKI Tohru

  United Graduate School of Agricultural Science, Gifu University

• Kawai Yoshiyuki

  Tokyo Dietitian Academy

• Kawai K

  Faculty Of Applied Biological Sciences Gifu University

• SAKURAI Hiroaki

  Department of Pathogenic Biochemistry, Toyama Medical and Pharmaceutical University

• TAKAMIZAWA Kazuhiro

  Department of Bioprocessing, Faculty of Agriculture, Gifu University

• Suzuki T

  Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University

• Kawai Y

  Kyoto Univ. Kyoto

• Sakurai Hidetoshi

  Nihon University

• Konishi Hiroaki

  Biochemical Research Institute, Nippon Menard Cosmetic Co., Ltd.

• SUZUKI TOHRU

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Konishi H

  The Division Of Cardiovascular Surgery Jichi Medical School

• Konishi H

  Biochemicalresearch Institute Nippon Menard Cosmetic Co. Ltd.

• Konishi Hiroaki

  Biosignal Research Center Kobe University

• Kawai Yoshifumi

  Biochemical Research Institute, Nippon Menard Cosmetic Co., Ltd.

• Horitsu Hiroyuki

  Department ofBiotechnology, Division of Bioresources Utilization, Faculty ofAgriculture,Gifu Univ.

• Kawai Keiichi

  Department of Biotechnology, Division of Bioresources Utilization, Faculty of Agriculture, Gifu Univ

• Takamizawa Kazuhiro

  Faculty of Applied Biological Sciences, Gifu University

• Sakurai H

  Nihon Univ. Fujisawa‐shi Jpn

• Sakurai Hiroaki

  Research Center Nippon Beet Sugar Mfg. Co. Ltd.

• Horitsu Hiroyuki

  Department Of Biotechnology Faculty Of Agriculture:(present Address)facculty Of Home Economics Depar

• Horitsu Hiroyuki

  Department Of Agricultural Chemistry Faculty Of Agriculture Gifu University

• Saito T

  Kanagawa Univ. Kanagawa Jpn

• Suzuki T

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Suzuki T

  Ihara & Company Ltd.

• So Tetsuya

  Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health

• Kawai Keiichi

  Faculty Of Applied Biological Sciences Gifu University

• Takamizawa K

  Faculty Of Applied Biological Sciences Gifu University

• Konishi Hiroaki

  Biochemical Institute Nippon Menard Cosmetic Co. Ltd.

• Takamizawa Kazuhiro

  Department Of Agriculture Gifu University

• Sakurai Hiroaki

  Department Of Bioscience Institute Of Natural Medicine Toyama University

• Kawai Keiichi

  Department Of Bioresources Utilization Faculty Of Agriculture Gifu University

• Kawai Y

  Faculty Of Fisheries Sciences Hokkaido University

• Konishi Hiroaki

  Division Of Cardiovascular Surgery Jichi Medical University

• KAWAI Keiichi

  Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University

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