Preparation and Some Properties of a Novel Maltopentaose-Forming Enzyme of a Pseudomonas species(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
A novel enzyme, of a Pseudomonas species, was purified by ammonium sulfate fractionation and column chromatographies on DEAE-Toyopearl 650M and Toyopearl HW-55s. The activity recovery was 87% at the ammonium sulfate fractionation step and 44% at the final step of the purification, respectively. The purified enzyme was homogeneous electrophoretically and its molecular weight was 72,000. The optimum pH and temperature were 6.5 and 55℃, respectively. The enzyme was stable up to 45℃ in the pH range of 6.5 to 9.0 for 1 hr, and thermostable in the presence of 2 mM CaCl_2 up to 50℃. The isoelectric point of the enzyme was 6.5. The enzyme activity was inhibited by metal ions such as Zn^<2+>, Cu^<2+>, Ag^+ and Hg^<2+>, and enhanced by Rb^+, Mg^<2+>, Co^<2+> and Ca^<2+>. The enzyme specifically produced maltopentaose from starch in the initial stage of the reaction, finally producing maltose and maltotriose. The enzyme did not act on glucose, maltose or maltotriose, and maltohexaose was considerably resistant to the enzyme's action. In the initial stage of hydrolysis, G_2 and G_5 were formed from G_7, and thus G_3+G_5 from G_8, G_4+G_5 from G_9, G_5+G_5 from G_<10>, and G_6+G_5 from G_<11>. Through the next action, G_4 and G_5 were degraded to G_2+G_2 and G_2+G_3, respectively. The enzyme action proceeds over the branching points of amylopectin to produce large amounts of G_2 and G_3, and a residual amount of oligosaccharides having less than 10 glucose units in the final stage of the reaction.
- 社団法人日本農芸化学会の論文
- 1990-01-23
著者
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Hashimoto H
Bio Research Corporation Of Yokohama
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Hashimoto Hitoshi
Materials Research Institute For Sustainable Development National Institute Of Advanced Industrial S
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Hara Koji
Bio Research Corporation Of Yokohama
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KOBAYASHI Shoichi
National Food and Research Institute, Ministry of Agriculture, Forestry and Fisheries
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Hashimoto H
Materials Research Institute For Sustainable Development National Institute Of Advanced Industrial S
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Hashimoto Hitoshi
Res. Inst. For Sustainable Materials National Inst. Of Advanced Industrial Sci. And Technol.
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Kobayashi S
Kyoto Univ. Kyoto Jpn
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OKEMOTO Hisashi
Ensuiko Sugar Refining Co., Ltd.
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HARA Kozo
Ensuiko Sugar Refining Co., Ltd.
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HASHIMOTO Hitoshi
Ensuiko Sugar Refining Co., Ltd.
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Okemoto Hisashi
Ensuiko Sugar Refining Co. Ltd.
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Hashimoto H
Department Of Biotechnology The University Of Tokyo
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Hashimoto Hitoshi
Ensuiko Sugar Refining Co. Ltd.
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