Cloning and Nucleotide Sequence of the Inulin Fructotransferase (DFA I-Producing)Gene of Arthrobacter globformis S14-3
スポンサーリンク
概要
- 論文の詳細を見る
A gene encoding an inulin fructotransferase (DFA I-producing) [EC 2. 4. 1. 200] from Arthrobacter globformis S14-3 was cloned and the nucleotides sequenced, for the first time. The sequence indicated that the native enzyme protein is composed of 392 amino acid residues. The native enzyme is an extracellar enzyme produced in the culture supernatant of A. globformis S14-3, but the nucleotide sequence of the gene lacks a sequence for signal peptide for secretion. The 1. 5-kb DNA fragment encoding the gene was found to produce the active enzyme in the culture supernatant of an E. coli clone, under the control of the lac promoter of pUC119.
- 社団法人日本農芸化学会の論文
- 1995-10-23
著者
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NAGATA Tadahiro
National Food Research Institute, Ministry of Agriculture, Forestry, and Fisheries
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KOBAYASHI Shoichi
National Food and Research Institute, Ministry of Agriculture, Forestry and Fisheries
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Kobayashi S
Kyoto Univ. Kyoto Jpn
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KASUMI Takafumi
National Food Research Institute
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Seki K
Chiba Univ. Chiba Jpn
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Seki Kiyohiko
Department Of Applied Biological Sciences Faculty Of Agriculture Saga University
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Kainuma K
Japan International Research Center For Agricultural Sciences Ministry Of Agriculture Forestry And F
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Nagata Tadahiro
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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Nagata Tadahiro
National Food Research Institute
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Haraguchi Kazutomo
National Food Research Institute, Ministry of Agriculture, Forestry, and Fisheries
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Seki Koji
Nihon Starch Co., Ltd.
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Kishimoto Mamoru
Nihon Starch Co., Ltd.
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Kainuma Keiji
Japan International Research Center for Agricultural Sciences, Ministry of Agriculture, Forestry, an
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Kishimoto Mamoru
Nihon Starch Co. Ltd.
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Haraguchi K
National Food Res. Inst. Ibaraki Jpn
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Haraguchi Kazutomo
National Food Research Institute
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Kasumi T
National Food Res. Inst. Ibaraki Jpn
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