Purification and Characterization of the Lytic Enzyme Produced by Streptomyces rutgersensis H-46
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概要
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Streptomyces rutgersensis H-46 isolated from soil produced a lytic enzyme noninducibly. This enzyme was active against Streptococcus faecalis, and termed SR-1 enzyme. SR-1 enzyme was purified from the cultivation broth to an electrophoretically homogeneous state as rod-shaped crystals. The molecular weight of SR-1 enzyme was estimated to be 22, 000 by gel filtration in the presence of 6 M guanidine-hydrochloride and SDS-polyacrylamide gel electrophoresis, and the isoelectric point was 9.19 by the isoelectric focusing method. SR-1 enzyme was relatively stable in a pH range of 47 and below 60°C, and showed maximum activity at pH 6.0, 48°C and at ionic strength 0.003 for lyophilized whole cells of S. faecalis. From analysis of digestion products with SR-1 enzyme, the enzyme was considered to be a kind of N-acetylmuramidase. The amino acid composition was also analyzed. The lytic spectra of the enzyme was investigated in 46 strains of species thought to be food putrefying microorganisms.
- 社団法人 日本農芸化学会の論文
著者
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Kubo Naoya
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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KASUMI Takafumi
National Food Research Institute
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Tsumura Nobuzo
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries:(present Office)taiy
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Hayashi Kiyoshi
National Food Research Institute
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