Characterization of a Thermostable Family 10 Endo-Xylanase (XynB) from Thermotoga maritima That Cleaves p-Nitrophenyl-β-D-Xlyloside

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概要

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• Thermotoga maritima MSB8 possesses two xylanase genes, xynA and xynB. The xynB gene was isolated from the genomic DNA of T.maritima, cloned, and expressed in Escherichia coli. XynB was purified to homogeneity by heat treatmnet, affinity chromatography and ion-exchange column chromatography. The purified enzyme produced a single band upon SDS-PAGE corresponding to a molecular mass of 42 kDa. At 70℃, the enzyme was stable between pH5.0 and pH11.4, and it was stable at temperatures of up to 100℃ from pH7.0 to pH8.5. At 50℃, XynB displayed an optimum pH of 6.14 and at this pH the temperature for optimal enzyme activity was 90℃. XynB exhibited broad substrate specificity and was highly active towards p-nitrophenyl-β-D-xylobioside with K_m and k_<cat> values of 0.0077 mM and 5.5s^<-1> respectively, at 30℃. It was also active towards p-nitrophenyl-β-D-xyloside. The initial product of the cleavage of p-nitrophenyl-β-D-xyloside was xylobiose, indicating that the major reaction in the cleavage was transglycosylation, not hydrolysis.

• 社団法人日本生物工学会の論文
• 2001-11-25

著者

• Kitaoka Motomitsu

  National Food Research Institute, National Agriculture and Food Research Organization

• Kitaoka M

  Enzyme Laboratory National Food Research Institute

• Kitaoka M

  Chubu Univ. Aichi Jpn

• Kitaoka Motomitsu

  National Food Res. Inst.

• Kitaoka Motomitsu

  Enzyme Laboratory National Food Research Institute National Agriculture And Food Research Organizati

• Hayashi K

  Enzyme Laboratory National Food Research Institute

• Hayashi Kiyoshi

  National Food Research Institute Ministry Of Agriculture Forestry And Fisheries

• Ahsan Mohammad

  National Food Research Institute

• Kitaoka Motomitsu

  National Food Research Institute

• ZHENGQIANG JIANG

  National Food Research Institute

• KOBAYASHI ATSUSHI

  National Food Research Institute

• LITE LI

  National Food Research Institute

• Kobayashi A

  National Food Research Institute

• Hayashi Kiyoshi

  National Food Research Institute

• Kitaoka Motomitsu

  National Agriculture And Food Research Organization National Food Research Institute

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