Adsorption Properties and Activities of Lipase on a Gold Substrate Modified by Self-assembled Monolayers(Analytical Chemistry)
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概要
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The adsorption properties, amount and specific activity of lipase D from Rhizopus delemar were investigated by employing a gold substrate modified with seven kinds of thiol monolayer. Quartz crystal microbalance measurements revealed that the amount of the enzyme adsorbed to the hydrophobic monolayers (e. g. benzenethiol) was much higher than that to the hydrophilic monolayers (e. g. 3-mercaptopropanoic acid). In contrast, lipase D adsorbed to the hydrophilic, 2-amino-l-ethanethiol monolayer showed the highest specific activity, the value being 300-fold higher than for the same enzyme dissolved in an aqueous medium.
- 社団法人日本農芸化学会の論文
- 2001-11-23
著者
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KOBAYASHI ATSUSHI
National Food Research Institute
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Sato Yukari
National Institute Of Advanced Industrial Science And Technology
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Mizutani F
Department Of Chemistry University Of Tsukuba
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Mizutani Fumio
National Institute Of Advanced Industrial Science And Technology
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Kobayashi Atsushi
National Institute Of Advanced Industrial Science And Technology
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