表面プラズモン共鳴免疫測定における高特異的吸着能と非特異的吸着抑制能を有するポリイオン混合薄膜修飾

概要

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The non-labeled and direct immunoassay of cytokine on a thin polyion complex film of poly-L-lysine and poly-styrenesulfonate was studied using a surface plasmon resonance sensor. The suppression of non-specific protein adsorption was investigated by changing the blend ratio of poly-L-lysine and poly-styrenesulfonate. With our film, the protein adsorption fell to less than 10% of that of an unmodified surface when the poly-L-lysine to poly-styrenesulfonate ratio was 4:6. We also measured the kinetic rates between tumor necrosis factor-α and anti-tumor necrosis factor-α antibodies, which were immobilized on the film. The binding constant was calculated to be 1.5×108 (M－1), which satisfies the binding constant level for a monoclonal antibody modified on a commercially available surface.
公益社団法人電気化学会の論文