Identification of the Putative Proton Donor Residue of Lacto-N-biose Phosphorylase (EC 2.4.1.211)
スポンサーリンク
概要
- 論文の詳細を見る
Two lacto-N-biose phosphorylase (LNBP) isozyme genes were cloned from Bifidobacterium bifidum JCM1254. Alignment of the amino acid sequences of LNBP and its homologs identified 24 completely conserved acidic amino acid residues. All single mutants of Bifidobacterium longum LNBP at residues other than D313N retained considerable activity, suggesting that Asp313 is the putative proton donor residue in LNBP.
- 社団法人 日本農芸化学会の論文
- 2007-06-23
著者
-
Kitaoka Motomitsu
National Food Res. Inst.
-
Nishimoto Mamoru
National Food Research Institute
-
Kitaoka Motomitsu
National Food Research Institute
-
Nishimoto Mamoru
National Food Res. Inst.
-
Kitaoka Motomitsu
National Agriculture And Food Research Organization National Food Research Institute
-
Nishimoto Mamoru
National Agriculture And Food Research Organization National Food Research Institute
関連論文
- Practical Preparation of D-Galactosyl-β1→4-L-rhamnose Employing the Combined Action of Phosphorylases
- The Role of Conserved Arginine Residue in Loop 4 of Glycoside Hydrolase Family 10 Xylanases
- Kinetic Studies on p-Nitrophenyl-cellobioside Hydrolyzing Xylanase from Cellvibrio gilvus
- 647 A putative proline iminopeptidase of Thermotoga maritime with leucine- and lysine-p-nitroanilide hydrolyzing activities
- Practical Preparation of Lacto-N-biose I, a Candidate for the Bifidus Factor in Human Milk
- Identification of the Putative Proton Donor Residue of Lacto-N-biose Phosphorylase (EC 2.4.1.211)
- Syntheses of 4-Methylumbelliferyl-β-D-Xylobioside and 5-Bromo-3-Indolyl-β-D-Xylobioside for Sensitive Detection of Xylanase Activity on Agar Plates
- Characterization of a Thermostable Family 10 Endo-Xylanase (XynB) from Thermotoga maritima That Cleaves p-Nitrophenyl-β-D-Xlyloside
- Analyses of Bifidobacterial Glycosidases Involved in the Metabolism of Oligosaccharides
- Kinetic Studies on the Hydrolysis of N-Acetylated and N-Deacetylated Derivatives of 4-Methylumbelliferyl Chitobioside by the Family 18 Chitinases ChiA and ChiB from Serratia Marcescens
- グライコシンターゼ化による反転型加水分解酵素のグリコシド合成触媒への変換
- Prebiotic Effect of Lacto-N-biose I on Bifidobacterial Growth
- Characterization of Bacillus halodurans α-Galactosidase Me14A Encoded by the mel4A Gene (BH2228)
- Practical Preparation of D-Galactosyl-β1→4-L-rhamnose Employing the Combined Action of Phosphorylases
- Structural Explanation for the Acquisition of Glycosynthase Activity
- Diversity and Similarity of Microbial Communities in Petroleum Crude Oils Produced in Asia
- Practical Preparation of Lacto-N-biose I, a Candidate for the Bifidus Factor in Human Milk
- Identification of the Putative Proton Donor Residue of Lacto-N-biose Phosphorylase (EC 2.4.1.211)
- Characterization of Glycosynthase Mutants Derived from Glycoside Hydrolase Family 10 Xylanases
- An Enzymatic Colorimetric Quantification of Orthophosphate
- Mutational Analysis of Fungal Family 11 Xylanases on pH Optimum Determination
- Interactions between Glycoside Hydrolase Family 94 Cellobiose Phosphorylase and Glucosidase Inhibitors
- Characterization of a Bacterial Laminaribiose Phosphorylase
- Effect of Lacto-N-biose I on the Antigen-specific Immune Responses of Splenocytes
- Characterization of the Cytosolic β-N-Acetylglucosaminidase from Bifidobacterium longum subsp. longum