Characterization of N-Acetylmuramidase M-1 of Streptomyces globisporus Produced by Escherichia coli BL21(DE3)pLysS
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概要
- 論文の詳細を見る
A gene of Streptomyces globisporus encoding N-acetylmuramidase M-1 was cloned into the pET26b vector and expressed in Escherichia coli BL21(DE3)pLysS. Maximal activity for the purified enzyme was observed at 55℃ with an optimal pH of 5.3, and N-bromosuccinimide strongly inhibited lytic activity even at a concentration of 0.01 mM. The enzyme showed N, О-diacetylmuramidase activity.
- 公益社団法人日本生物工学会の論文
- 2001-11-25
著者
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Hayashi K
Enzyme Laboratory National Food Research Institute
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HAYASHI Kiyoshi
Enzyme Applications Laboratory, National Food Research Institute
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Seo Hyo
Enzyme Laboratory National Food Research Institute:department Of Bioscience Faculty Of Bioresources
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Ohmiya Kunio
Department Of Bioresources School Of Bioresources Mie University
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Shimonishi Tsuyoshi
Enzyme Laboratory National Food Research Institute
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