Characterization of N-Acetylmuramidase M-1 of Streptomyces globisporus Produced by Escherichia coli BL21(DE3)pLysS

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概要

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• A gene of Streptomyces globisporus encoding N-acetylmuramidase M-1 was cloned into the pET26b vector and expressed in Escherichia coli BL21(DE3)pLysS. Maximal activity for the purified enzyme was observed at 55℃ with an optimal pH of 5.3, and N-bromosuccinimide strongly inhibited lytic activity even at a concentration of 0.01 mM. The enzyme showed N, О-diacetylmuramidase activity.

• 公益社団法人日本生物工学会の論文
• 2001-11-25

著者

• Hayashi K

  Enzyme Laboratory National Food Research Institute

• HAYASHI Kiyoshi

  Enzyme Applications Laboratory, National Food Research Institute

• Seo Hyo

  Enzyme Laboratory National Food Research Institute:department Of Bioscience Faculty Of Bioresources

• Ohmiya Kunio

  Department Of Bioresources School Of Bioresources Mie University

• Shimonishi Tsuyoshi

  Enzyme Laboratory National Food Research Institute

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