Substrate Specificity of the N, 6-ο-Diacetylmuramidase from Streptomyces globisporus

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概要

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• We found that the N,6-ο-diacetylmuramidase from Streptomyces globisporus (M-1) hydrolyzed the cell walls from Micrococcus lysodeikticus and Staphylococcus aureus. In contrast, hen egg white lysozyme (HEWL) was only able to hydrolyze the cell walls from M. lysodeikticus. 6-ο-Acetylation of the muramoyl moieties, as found in the S. aureus cell walls, did not inhibit the activity of the M-1 enzyme whereas it was sufficient to inhibit HEWL. The disaccharide GlcNAc-MurNAc was not observed in the M. lysodeikticus cell wall hydrolyzate produced by the M-1, indicating that M-1 acts on the MurNAc moiety which are linked by peptides at the lactyl groups of the MurNAc moiety. M-1 displays both N-acetylmuramidase and N,6-ο-diacerylmuramidase activity and has a different substrate specificity from HEWL.

• 社団法人日本生物工学会の論文
• 2003-03-25

著者

• Kitaoka Motomitsu

  National Food Research Institute, National Agriculture and Food Research Organization

• Ohmiya K

  Graduate School Of Bioresources Mie Univ.

• Ohmiya Kunio

  Department Of Food Science And Technology Faculty Of Agriculture Nagoya University

• KITAOKA Motomitsu

  Enzyme Laboratory, National Food Research Institute, National Agriculture and Food Research Organiza

• Kitaoka M

  Enzyme Laboratory National Food Research Institute

• Kitaoka M

  Chubu Univ. Aichi Jpn

• Kitaoka Motomitsu

  Enzyme Laboratory National Food Research Institute National Agriculture And Food Research Organizati

• Hayashi K

  Enzyme Laboratory National Food Research Institute

• HAYASHI Kiyoshi

  Enzyme Applications Laboratory, National Food Research Institute

• Kitaoka Motomitsu

  Enzyme Laboratory National Food Research Institute

• 切畑 光統

  大阪府立大学 農学部

• Kitaoka Motomitsu

  Department Of Biological Chemistry Chubu University

• Seo Hyo

  Enzyme Laboratory National Food Research Institute:department Of Bioscience Faculty Of Bioresources

• Ohmiya Kunio

  Department Of Bioresources School Of Bioresources Mie University

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