An Investigation of the pH-Activity Relationships of Cex, a Family 10 Xylanase from Cellulomonas fimi : Xylan Inhibition and the Influence of Nitro-Substituted Aryl-β-D-Xylobiosides on Xylanase Activity

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概要

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• The kinetic parameters of Cex, a family 19 xylanase from Cellulomonas fimi, were determined at various pH levels using soluble birchwood xylan (BWX) as a natural polymeric substrate along with three other synthetic aryl-β-D-xylobioside substrates. Using BWX, a high level of substrate inhibition was observed which increased with decreasing pH. In contrast, typical Michaelis-Menten-type profiles were obtained using the three aryl-β-D-xylobiosides as substrates. The k_<cat> values determined using o-nitrophenyl-β-D-xylobioside did not change as the pH increased, whereas the k_<cat>, values obtained with BWX, phenyl-β-D-xylobioside and p-nitrophenyl-β-D-xylobioside decreased, suggesting that the presence of an ortho nitro group affects the activity displayed by Cex. These differences were not observed with XynB from Clostridium stercorarium F9, a member of the same family of xylanases as Cex. These results indicate that a careful evaluation is required when employing substituted aryl-β-D-xylobiosides in the characterization of xylanases.

• 社団法人日本生物工学会の論文
• 2002-03-25

著者

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• KITAOKA Motomitsu

  Enzyme Laboratory, National Food Research Institute, National Agriculture and Food Research Organiza

• Kitaoka M

  Enzyme Laboratory National Food Research Institute

• Kitaoka M

  Chubu Univ. Aichi Jpn

• Miyatake K

  Osaka Prefecture Univ. Osaka Jpn

• Hayashi K

  Enzyme Laboratory National Food Research Institute

• HAYASHI Kiyoshi

  Enzyme Applications Laboratory, National Food Research Institute

• SAKKA Kazuo

  Faculty of Bioresources, Mie University

• Kitaoka Motomitsu

  Enzyme Laboratory National Food Research Institute

• MATSUDA Kazuhiko

  Laboratory of Plant Pathology and Biotechnology and Laboratory of Pesticide Chemistry

• Kirihata Mitsunori

  Department Of Agricultural Chemistry Faculty Of Agriculture University Of Osaka Prefecture

• 切畑 光統

  大阪府立大学 農学部

• Kirihata Mitsunori

  Graduate School Of Life And Environmental Science Osaka Prefecture University

• Honda Yuji

  National Food Research Institute 2-1-12 Kannondai

• Honda Y

  Osaka Prefecture Univ. Sakai Jpn

• Sakka Kazuo

  Faculty Of Bioresources Mie University

• HONDA YUJI

  Enzyme Laboratory, National Food Research Institute

• Honda Yuji

  Laboratory Of Bioorganic Chemistry College Of Agriculture Osaka Prefecture University

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