Cloning and Sequencing of the Gene Encoding Chitinase ChiA from Xanthomonas sp.Strain AK and Some Properties of ChiA

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概要

• 論文の詳細を見る

• The chiA gene encoding chitinase A was cloned into Escherichia coli from Xanthomonas sp.strain AK and its nucleotide sequence was determined. The structural gene consists of 1788 bp encoding 596 amino acids with a predicted molecular weight of 62, 122. The deduced ChiA is a modular enzyme composed of an N-terminal signal peptide and four domains in the following order: a chitin-binding domain, two fibronectin type III domains, and a family 18 catalytic domain. ChiA purified from the recombinant E.coli had temperature and pH optima at 35℃ and 4.5, respectively. The K_m and V_<max> values for colloidal chitin were estimated to be 1.8 mg/ml and 8.7 μmol/min/mg, respectively.

• 社団法人日本生物工学会の論文
• 1998-12-25

著者

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• KIMURA Tetsuya

  Faculty of Bioresources, Mie University

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Kimura Tetsuya

  National Center For Seeds And Seedlings

• Karita Shuichi

  Faculty of Bioresources, Mie University

• SAKKA Kazuo

  Faculty of Bioresources, Mie University

• Sakka Kazuo

  Faculty Of Bioresources Mie University

• Karita Shuichi

  Faculty Of Bioresources Mie University

• Kawano Akihiro

  Faculty Of Bioresources Mie University

• SUKHUMAVASI JIRAPORN

  Thailand Institute of Scientific and Technological Research

• KUSAKA RYO

  Faculty of Bioresources, Mie University

• Kusaka Ryo

  Faculty Of Bioresources Mie University

• Sukhumavasi J

  Thailand Institute Of Scientific And Technological Research

• Kimura Tetsuya

  Faculty Of Bioresources Mie University

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