The Involvement of Two Specific Arginine Residues in the Action of HM-1 Killer Toxin was Deduced from Site-Directed Mutagenesis

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概要

• 論文の詳細を見る

• Each of the four arginine residues in the HM-1 killer toxin was replaced by alanine using site-directed mutagenesis. The polymerase chain reaction (PCR)-constructed mutant gene was successfully expressed in HM-1 toxin resistant Saccharomyces cereviasiae. Among four HM-1 toxin analogues, R82A HM-1 toxin and R86A HM-1 toxin lost killer activity, while R61A HM-1 toxin and R85A HM-1 toxin retained activity. These results strongly indicate the importance of the arginine residues at positions 82 and 86 which are located in the C-terminal region of the HM-1 toxin for the action of killer activity.

• 社団法人日本薬学会の論文
• 2000-08-01

著者

• Furuichi Y

  Genecare Research Institute

• Furuichi Yasuhiro

  新潟薬科大学 薬学部生化学

• Furuichi Yasuhiro

  ジーンケア研究所

• Furuichi Yasuhiro

  Genecare Research Institute Co. Ltd.

• KOMIYAMA Tadazumi

  Department of Biochemistry, Niigata College of Pharmacy

• KIMURA Tetsuya

  Faculty of Bioresources, Mie University

• SHIRAI Tarou

  Department of Biochemistry, Niigata College of Pharmacy

• FURUICHI Yasuhiro

  AGENE Research Institute

• Shirai T

  Niigata Coll. Pharmacy Niigata Jpn

• Furuichi Yasuhiro

  Genecare Research Institute Co. Ltd.:core Research For Evolution Science And Technology Japan Scienc

• Kimura T

  Okayama Univ. Okayama Jpn

• Koide T

  Laboratory Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Nagoya City University

• Komiyama T

  Department Of Biochemistry Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And App

• Komiyama Tadazumi

  Department Of Biochemistry Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And App

• Kimura Tetsuya

  Faculty Of Bioresources Mie University

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