Recombinant Single-Chain Anti-idiotypic Antibody : An Effective Fungal β-1,3-Glucan Synthase Inhibitor(Microbiology)
スポンサーリンク
概要
- 論文の詳細を見る
Recombinant single-chain fragment variable anti-idiotypic antibodies were produced to represent the internal image of HM-1 killer toxin and were used as novel and effective antifungal agents to inhibit in vitro β-1, 3-glucan synthase and cell growth. The mechanism of cytocidal activity of anti-idiotypic antibodies was investigated and was compared with the actions of aculeacin A and papulacandin B, the most common antibiotics acting as β-1, 3-glucan synthase inhibitors. The degree of inhibition of β-1, 3-glucan synthase by both antibodies and antibiotics were examined for yeasts Saccharomyces cerevislae A451, Cryptococcus albidus NBRC 0612 and Candida albicans IFM 40215. Although the mechanism of actions of the anti-idiotypic antibodies and antibiotics seems identical, the IC_<50> values for the various yeasts used in this study confirmed that anti-idiotypic antibodies could be used as more effective fungal β-1, 3-glucan synthase inhibitors than those of antibiotics.
- 社団法人日本薬学会の論文
- 2006-09-01
著者
-
FURUICHI Yasuhiro
GeneCare (previously AGENE) Research Institute
-
Furuichi Y
Genecare Research Institute
-
Furuichi Yasuhiro
新潟薬科大学 薬学部生化学
-
Furuichi Yasuhiro
Genecare Research Institute
-
Furuichi Yasuhiro
Genecare Research Institute Co. Ltd.
-
KOMIYAMA Tadazumi
Department of Biochemistry, Niigata College of Pharmacy
-
SELVAKUMAR Dakshnamurthy
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and A
-
KARIM Nurul
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and A
-
MIYAMOTO Masahiko
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and A
-
Miyamoto M
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And App
-
Karim Nurul
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And App
-
Koide T
Laboratory Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Nagoya City University
-
Komiyama T
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And App
-
Komiyama Tadazumi
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And App
-
Selvakumar Dakshnamurthy
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And App
関連論文
- Terminal Telomere Repeats Are Actually Short in Telomerase-Negative Immortal Human Cells(Highlighted paper selected by Editor-in-chief)(Biochemistry/Molecular Biology)
- Differential Cytotoxicity of Anticancer Agents in Pre- and Post-Immortal Lymphoblastoid Cell Lines(Pharmacology)
- Effects of Idebenone on Cyanide-Resistant Respiration of the Mitochondria Isolated from Hansenula anomala(Biological Chemistry)
- Expression of Mitochondrial Cyanide-resistant Respiration in the Absence of Respiratory Inhibitors in Hansenula anomala(Microbiology & Fermentation Industry)
- Recombinant Single-Chain Anti-idiotypic Antibody : An Effective Fungal β-1,3-Glucan Synthase Inhibitor(Microbiology)
- Identification and Characterization of a Neutralizing Monoclonal Antibody for the Epitope on HM-1 Killer Toxin
- Inhibition of β-1,3-Glucan Synthase and Cell Growth of Cryptococcus species by Recombinant Single-chain Anti-idiotypic Antibodies
- Monoclonal Antibodies and Sandwich ELISA for Quantitation of HM-1 Killer Toxin (Analytical Biochemistry)
- Round Shape Enlargement of the Yeast Spheroplast of Saccharomyces cerevisiae by HM-1 Toxin
- The Involvement of Two Specific Arginine Residues in the Action of HM-1 Killer Toxin was Deduced from Site-Directed Mutagenesis
- Dynamic Responses to Acute Heat Stress between 34 ℃ and 38.5 ℃, and Characteristics of Heat Stress Response in Mice
- Identification of a Key Amino Acid of the Human 5-HT_ Serotonin Receptor Important for Sarpogrelate Binding
- Selectivity of Sarpogrelate to the Recombinant Human 5-HT_ Receptors as Assessed by Molecular Pharmacology and Molecular Modeling
- Anticancer activity of RecQL1 helicase siRNA in mouse xenograft models
- Induction of mitotic cell death in cancer cells by small interference RNA suppressing the expression of RecQL1 helicase
- Increased Chemotherapeutic Activity of Camptothecin in Cancer Cells by siRNA-Induced Silencing of WRN Helicase(Molecular and Cell Biology)
- Hyaline vascular-type Castleman's disease in the hepatoduodenal ligament : Report of a case
- Innate Apoptosis of Human B Lymphoblasts Transformed by Epstein-Barr Virus : Modulation by Cellular Immortalization and Senescence
- Alanine-Scanning Mutagenesis of HM-1 Killer Toxin and the Essential Residues for Killing Activity
- Leishmanicidal Effect of Curcumin in Vitro
- Trypanocidal Effects of Curcumin in Vitro
- Formation of Reactive Oxygen Intermediates Might be Involved in the Trypanocidal Activity of Gallic Acid
- Oral and Intraperitoneal treatment of Trypanosoma brucei brucei with a combination of ascofuranone and glycerol in mice
- Beta-Blockers Show Inverse Agonism to a Novel Constitutively Active Mutant of β_1-Adrenoceptor
- Pharmacological and Molecular Characterization of Novel SWR-Compounds for β-Adrenergic Receptors as assessed by Molecular Modeling, Site-Directed Mutagenesis, Binding Affinity and Functional Activity
- Identification of Amino Acid Residues Important for Sarpogrelate Binding to the Human 5-Hydroxytryptamine_ Serotonin Receptor
- Site-Directed Mutagenesis of the Serotonin 5-Hydroxytryptamine_ Receptor : Identification of Amino Acids Responsible for Sarpogrelate Binding(Pharmacology)
- Effects of Estrogenic Agents 17β-Estradiol (E2) and Bisphenol A on the Expression of RecQ DNA Helicases in Mammary Tumor MCF-7 Cells
- Mitochondrial and Nuclear Localization of Human Pif1 Helicase(Molecular and Cell Biology)
- Modulation of the Expression of Bloom Helicase by Estrogenic Agents(Molecular and Cell Biology)
- Significant elevation of IgG anti-WRN (RecQ3 RNA/DNA helicase) antibody in systemic sclerosis
- DNA Damage Caused by Bisphenol A and Estradiol through Estrogenic Activity(Highlighted paper selected by Editor-in-chief,Analytical Biochemistry)
- Quantitative Analysis of Werner Helicase Activity Using the Single-Molecule Fluorescence Detection System MF10S(Analytical Biochemistry)
- Molecular biology of Werner syndrome
- A Case of Congenital Photosensitivity, Hair Loss, Growth Failure and Pulmonary Stenosis: Relationship with Rothmund-Thomson Syndrome
- Electrically Induced Orientation of Poly(silane)s
- Identification of a Key Amino Acid of the Human 5-HT2B Serotonin Receptor Important for Sarpogrelate Binding
- Effects of Peptidase Inhibitors, jp-Ala2, Met5]-Enkephalinamide and Antiserum to Methionine-Enkephalin Microinjected Into the Caudal Periaqueductal Gray on Morphine Withdrawal in Rats
- Biodistribution of Gadolinium Incorporated in Lipid Emulsions Intraperitoneally Administered for Neutron-Capture Therapy with Tumor-Bearing Hamsters
- Preparation of Gadolinium-Containing Emulsions Stabilized with Phosphatidylcholine-Surfactant Mixtures for Neutron-Capture Therapy
- Dry grinding of chitosan powder by a planetary ball mill
- Action Properties of HYI Killer Toxin from Williopsis saturnus var. saturnus, and Antibiotics, Aculeacin A and Papulacandin B
- Design and Preparation of Gadolinium-Reservior Microcapsules for Neutron-Capture Therapy by Measu of the Wurster Process
- Role of Werner syndrome gene product helicase in carcinogenesis and in resistance to genotoxins by cancer cells
- PREVALENCE OF WERNER SYNDROME GENE MUTATIONS IN THE JAPANESE POPULATION : A GENETIC EPIDEMIOLOGICAL STUDY
- A case of intracranial hemorrhage in utero : Abstracts of Papers Presented at the Thirty-Third Annual Meeting of the Japanese Teratology Society Nagoya, Japan July 21-23, 1993
- Pore Formation on Proliferating Yeast Saccharomyces Cerevisiae Cell Buds by HM-1 Killer Toxin
- Steroid Monooxygenase of Rhodoccus rhodochrous: Sequencing of the Genomic DNA, and Hyperexpression, Purification, and Characterization of the Recombinant Enzyme
- PHENOTYPES OF CELLS WITH A WRN GENE MUTATION
- Differential Effects of Cytotoxic Drugs on Mortal and Immortalized B-Lymphoblastoid Cell Lines from Normal and Werner's Syndrome Patients
- ESTIMATION OF THE PHYSICAL DISTANCE BETWEEN MAJOR GENOMIC MARKERS IN THE WERNER SYNDROME LOCUS (8p11.2-12) BY DUAL-COLOR FISH ANALYSIS
- Different buffer effects in selecting HM-1 killer toxin single-chain fragment variable anti-idiotypic antibodies
- Beta-Blockers Show Inverse Agonism to a Novel Constitutively Active Mutant of .BETA.1-Adrenoceptor
- Pore Formation on Proliferating Yeast Saccharomyces cerevisiae Cell Buds by HM-l Killer Toxin.