Mitochondrial and Nuclear Localization of Human Pif1 Helicase(Molecular and Cell Biology)
スポンサーリンク
概要
- 論文の詳細を見る
Yeast Pif1 DNA helicase is the prototype member of a helicase subfamily participating in the maintenance of telomere, ribosome, and mitochondria DNAs. The Pif1 DNA helicase family is highly conserved from yeast to human, but the biochemical nature of human homologues remains to be clarified. To this end, we investigated the transcriptional unit of human Pif1 gene and its encoded protein hPif1. The results showed that the hPif1 gene product has at least two isoforms consisting of the conserved helicase motif and differential C-terminal regions derived from alternative splicing of the gene transcript. Deletion mutant analysis showed that Pif1 helicase has nuclear localization signal and mitochondria targeting signal at the N-terminal and C-terminal regions, respectively. In HeLa cells, hPif1 helicase expression was induced by the release of cells from serum starvation, suggesting that hPif1 has roles in the S phase. Consistently, the down regulation of the hPif1 helicase by RNA interference with siRNA caused a cell cycle delay at the S phase. These findings suggest that hPif1 in the nucleus may be involved in chromosome maintenance in association with DNA replication, while the function of hPifl remains to be clarified.
- 公益社団法人日本薬学会の論文
- 2007-09-01
著者
-
Furuichi Y
Genecare Research Institute
-
Furuichi Yasuhiro
ジーンケア研究所
-
Furuichi Yasuhiro
Genecare Research Institute
-
FUTAMI Kazunobu
GeneCare Research Institute
-
SHIMAMOTO Akira
GeneCare Research Institute
-
FURUICHI Yasuhiro
Gene Care (previously AGENE) Research Institute
-
FUTAMI Kazunobu
Gene Care Research Institute Co., Ltd.
-
SHIMAMOTO Akira
Gene Care Research Institute Co., Ltd.
-
Furuichi Yasuhiro
Genecare Research Institute Co. Ltd.:core Research For Evolution Science And Technology Japan Scienc
関連論文
- Terminal Telomere Repeats Are Actually Short in Telomerase-Negative Immortal Human Cells(Highlighted paper selected by Editor-in-chief)(Biochemistry/Molecular Biology)
- Recombinant Single-Chain Anti-idiotypic Antibody : An Effective Fungal β-1,3-Glucan Synthase Inhibitor(Microbiology)
- Identification and Characterization of a Neutralizing Monoclonal Antibody for the Epitope on HM-1 Killer Toxin
- Inhibition of β-1,3-Glucan Synthase and Cell Growth of Cryptococcus species by Recombinant Single-chain Anti-idiotypic Antibodies
- Monoclonal Antibodies and Sandwich ELISA for Quantitation of HM-1 Killer Toxin (Analytical Biochemistry)
- Round Shape Enlargement of the Yeast Spheroplast of Saccharomyces cerevisiae by HM-1 Toxin
- The Involvement of Two Specific Arginine Residues in the Action of HM-1 Killer Toxin was Deduced from Site-Directed Mutagenesis
- Anticancer activity of RecQL1 helicase siRNA in mouse xenograft models
- Induction of mitotic cell death in cancer cells by small interference RNA suppressing the expression of RecQL1 helicase
- Increased Chemotherapeutic Activity of Camptothecin in Cancer Cells by siRNA-Induced Silencing of WRN Helicase(Molecular and Cell Biology)
- Innate Apoptosis of Human B Lymphoblasts Transformed by Epstein-Barr Virus : Modulation by Cellular Immortalization and Senescence
- Alanine-Scanning Mutagenesis of HM-1 Killer Toxin and the Essential Residues for Killing Activity
- Effects of Estrogenic Agents 17β-Estradiol (E2) and Bisphenol A on the Expression of RecQ DNA Helicases in Mammary Tumor MCF-7 Cells
- Mitochondrial and Nuclear Localization of Human Pif1 Helicase(Molecular and Cell Biology)
- Modulation of the Expression of Bloom Helicase by Estrogenic Agents(Molecular and Cell Biology)
- Significant elevation of IgG anti-WRN (RecQ3 RNA/DNA helicase) antibody in systemic sclerosis
- DNA Damage Caused by Bisphenol A and Estradiol through Estrogenic Activity(Highlighted paper selected by Editor-in-chief,Analytical Biochemistry)
- Quantitative Analysis of Werner Helicase Activity Using the Single-Molecule Fluorescence Detection System MF10S(Analytical Biochemistry)
- Molecular biology of Werner syndrome
- A Case of Congenital Photosensitivity, Hair Loss, Growth Failure and Pulmonary Stenosis: Relationship with Rothmund-Thomson Syndrome
- Action Properties of HYI Killer Toxin from Williopsis saturnus var. saturnus, and Antibiotics, Aculeacin A and Papulacandin B
- A Method of Measuring Physical Properties from Stress Fringe Values
- Study on the Development of Photoelastic Experimental Composite Material : Development of Orthotropic Material
- Role of Werner syndrome gene product helicase in carcinogenesis and in resistance to genotoxins by cancer cells
- PREVALENCE OF WERNER SYNDROME GENE MUTATIONS IN THE JAPANESE POPULATION : A GENETIC EPIDEMIOLOGICAL STUDY
- Pore Formation on Proliferating Yeast Saccharomyces Cerevisiae Cell Buds by HM-1 Killer Toxin
- PHENOTYPES OF CELLS WITH A WRN GENE MUTATION
- Differential Effects of Cytotoxic Drugs on Mortal and Immortalized B-Lymphoblastoid Cell Lines from Normal and Werner's Syndrome Patients
- ESTIMATION OF THE PHYSICAL DISTANCE BETWEEN MAJOR GENOMIC MARKERS IN THE WERNER SYNDROME LOCUS (8p11.2-12) BY DUAL-COLOR FISH ANALYSIS
- Different buffer effects in selecting HM-1 killer toxin single-chain fragment variable anti-idiotypic antibodies