Effects of Estrogenic Agents 17β-Estradiol (E2) and Bisphenol A on the Expression of RecQ DNA Helicases in Mammary Tumor MCF-7 Cells
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概要
- 論文の詳細を見る
17β-Estradiol (E2) and bisphenol A (BPA) damage DNA in estrogen receptor (ER)-positive human mammary tumor MCF-7 cells. The effect of E2 and BPA is associated with increased expression of Bloom helicase (BLM) and formation of nuclear foci consisting of BLM and γ-H2AX at double stranded break regions. BLM is one of five human RecQ helicases that participate in the maintenance of genomic stability. This study investigated if the expressions of RecQ helicases Werner syndrome helicase (WRN), Rothmund-Thomson syndrome helicase (RTS), RecQ5 helicase (RecQ5), RecQ protein-like 1 helicase (RecQL1, also known as RecQ1) and BLM helicase are affected in MCF-7 cells by treatment with estrogenic agents. The expression of RTS mRNA significantly increased although the expression in RTS protein only slightly increased. The expressions of WRN, RecQ5 and RecQL1 were almost unaffected both at mRNA and protein levels. These data suggest that the expression of human RecQ helicases is differentially regulated depending on the tissues and cells. We showed that some helicase expressions are regulated depending on the DNA damage.
- 公益社団法人日本薬学会の論文
- 2009-02-01
著者
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FURUICHI Yasuhiro
GeneCare (previously AGENE) Research Institute
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Furuichi Y
Genecare Research Institute
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Furuichi Yasuhiro
ジーンケア研究所
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Furuichi Yasuhiro
Genecare Research Institute
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FUTAMI Kazunobu
GeneCare Research Institute
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ISO Takako
GeneCare Research Institute Co., Ltd.
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Iwamoto Teruaki
Core Research for Evolution Science and Technology, Japan Science and Technology Agency
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Iwamoto Teruaki
Core Research For Evolution Science And Technology Japan Science And Technology Agency:division Of M
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Furuichi Yasuhiro
Genecare Research Institute Co. Ltd.:core Research For Evolution Science And Technology Japan Scienc
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Iso Takako
Genecare Research Institute Co. Ltd.:core Research For Evolution Science And Technology Japan Scienc
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Futami Kazunobu
Genecare Research Institute Co. Ltd.
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