Cloning, Sequencing, and Expression of the Gene Encoding the Clostridium stercorarium α-Galactosidase Aga36A in Escherichia coli(Microbiology & Fermentation Technology)

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概要

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• The α-galactosidase gene aga36A of Clostridium stercorarium F-9 was cloned, sequenced, and expressed in Escherichia coli. The aga36A gene consists of 2,208 nucleotides encoding a protein of 736 amino acids with a predicted molecular weight of 84,786. Aga36A is an enzyme classified in family 36 of the glycoside hydrolases and showed sequence similarity with some enzymes of family 36 such as Geobacillus (formerly Bacillus) stearothermophilus GalA (57%) and AgaN (52%). The enzyme purified from a recombinant E. coli is optimally active at 70℃ and pH 6.0. The enzyme hydrolyzed raffinose and guar gum with specific activities of 3.0 U/mg and 0.46 U/mg for the respective substrates.

• 社団法人日本農芸化学会の論文
• 2003-10-23

著者

• Ohmiya K

  Graduate School Of Bioresources Mie Univ.

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• KIMURA Tetsuya

  Faculty of Bioresources, Mie University

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Kimura Tetsuya

  National Center For Seeds And Seedlings

• SAKKA Kazuo

  Faculty of Bioresources, Mie University

• Sun Jia-lin

  Center For Molecular Biology And Genetics Mie University:department Of Bioresources Graduate School

• Sun Jia-lin

  Graduate School Of Bioresources Mie Univ.

• Sakka Kazuo

  Faculty Of Bioresources Mie University

• Kimura Tetsuya

  Graduate School Of Bioresources Mie University

• Sun Jialin

  Graduate School Of Bioresources Mie University

• Kimura Tetsuya

  Faculty Of Bioresources Mie University

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