Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene
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概要
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The Paenibacillus curdlanolyticus xyn10B gene encoding a family-10 xylanase was cloned and expressed in Escherichia coli, and the recombinant enzyme rXyn10B was characterized. Immunological analysis suggested that Xyn10B is an intracellular enzyme. rXyn10B hydrolyzed birch-wood xylan and xylooligosaccharides to produce mainly xylobiose, suggesting that it is an endoxylanase. Its properties were significantly different from those of some homologous enzymes.
著者
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Kajino T
Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.
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Sudo Maya
Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University
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Sakka Makiko
Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University
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Kimura Tetsuya
Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University
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RATANAKHANOKCHAI Khanok
School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi
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SAKKA Kazuo
Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University
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