Peptide Mapping and Assessment of Cryoprotective Activity of 26/27-kDa Dehydrin from Soybean Seeds(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
To characterize the molecular weight diversity of seed dehydrin among soybean cultivars, 26/27-kDa soybean dehydrins were purified and compared in peptide mapping patterns, partial amino acid sequences, and cryoprotective activity on enzyme. In reverse phase chromatograms of their trypsin digests, we detected several distinctive peaks, one of which was attributed to a part of the internal glycine-rich region. Partial amino acid sequences of peptide fragments from trypsin and S. aureus V8 protease cleavage were found to be identical to the Mat9 translation. The CP_<50> of purified 26/27-kDa dehydrins were estimated to be 0.30 and 0.11 μM, respectively.
- 社団法人日本農芸化学会の論文
- 2003-08-23
著者
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Matsukura Ushio
National Agriculture and Food Research Organization (NARO), National Institute of Crop Science
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MOMMA Michiko
National Food Research Institute
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Kaneko Shigenobu
National Institute Of Crop Science (nics)
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Kaneko Shigenobu
National Food Research Institute:(present Address)national Institute Of Crop Science
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Kaneko Shigenobu
National Agriculture Research Center
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Haraguchi Kazutomo
National Food Research Institute, Ministry of Agriculture, Forestry, and Fisheries
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Matsukura Ushio
National Agriculture And Food Research Organization (naro) National Institute Of Crop Science:nation
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Matsukura Ushio
National Food Research Institute:(present Address)national Institute Of Crop Science
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Haraguchi Kazutomo
National Food Research Institute
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