Effect of Gene Disruption of Succinate Dehydrogenase on Succinate Production in a Sake Yeast Strain
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概要
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Succinate dehydrogenase(SDH) of Saccharomyces cerevisiae consists of four subunits encoded by the SDH1, SDH2, SDH3, and SDH4 genes. We determined the effect of SDH deficiency on the productivity of organic acids in a sake yeast strain Kyokai no.9. The SDH activity of single disruptants was retained at 30-90% of that of the wild-type strain, but tha activity disappeared in double disruptants ot the SDH1 and SDH2 or SDH1b(the SDH1 homologue) genes. Two double disruptants showed no growth on a medium containing glycerol as the sole carbon source, while the single disruptants could utilize glycerol. These results indicate that double disruption of the SDH1 and SDH2 or SDH1b genes is required for complete loss of SDH activity and that the SDH1b gene compensates for the function of the SDH1 gene. The sdh1b sdh1b disruptant showed a marked increase in succinate productivity of up to 1.9-fold along with a decrease in malate productivity relative to the wild-type strains under shaking conditions. Under both static and sake brewing conditions, the productivity of these organic acids in the disruptants was virtually unchanged from that in the wild-type strain. Furthermore, SDH activity was undetectable in the wild-type and the disrupted strains under static conditions. These results suggest that SDH activity contributes to succinate production under shaking conditions, but not under static and sake brewing conditions.
- 社団法人日本生物工学会の論文
- 2000-12-25
著者
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TAKAGI Hiroshi
Department of Surgery II, University School of Medicine
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HIRAI TAKANORI
Komaki Municipal Hospital
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NAKAMORI Shigeru
Department of Bioscience, Fukui Prefectural University
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Takagi H
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University:(present Address)
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Takagi Hiroshi
Second Department Of Surgery Nagoya University
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Kubo Yoshito
Fukui Prefectural Food Processing Research Institute
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Nakamori Shigeru
Dept. Biosci. Fukui Pref. Univ.
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Nakamori Shigeru
Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University
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Takagi H
Department Of Bioscience Fukui Prefectural University
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Takagi Hiroshi
Department Of Medicine And Molecular Science Gunma University Graduate School Of Medicine
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Nakamori S
Department Of Bioscience Fukui Prefectural University
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Takagi H
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
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Takagi Hiroshi
Department Of Bioscience Fukui Prefectural University
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Takagi Hiroshi
Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University
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