Efficient Selection for Thermostable Protease in Themus thermophilus
スポンサーリンク
概要
- 論文の詳細を見る
An efficient procedure was established to select for thermostable proteases in an extreme thermophile, Themus thermophilus. A non-protease-secreting mutant derived from T. thermophilus TH125 was used as host and the expression plasmid for aqualysin I from T. aquaticus YT-1 was constructed as a source of thermostable protease. T. thermophilus cells harboring the recombinant plasmid produced active aqualysin I into the medium and were able to grow on a minimal medium containing milk casein as the sole source of carbon and nitrogen.
- 社団法人日本農芸化学会の論文
- 2000-04-23
著者
-
TAKAGI Hiroshi
Department of Surgery II, University School of Medicine
-
NAKAMORI Shigeru
Department of Bioscience, Fukui Prefectural University
-
Hoshino Takayuki
Institute Of Applied Biochemistry University Of Tsukuba
-
SUZUMURA Akitoshi
Department of Advanced Materials Science, The University of Tokyo
-
Nakamori Shigeru
Dept. Biosci. Fukui Pref. Univ.
-
Nakamori Shigeru
Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University
-
Takagi Hiroshi
Department Of Medicine And Molecular Science Gunma University Graduate School Of Medicine
-
HASUURA Yoshiyuki
Department of Bioscience, Fukui Prefectural University
-
Nakamori S
Department Of Bioscience Fukui Prefectural University
-
Suzumura Akitoshi
Department Of Advanced Materials Science The University Of Tokyo
-
Suzumura Akitoshi
Department Of Bioscience Fukui Prefectural University
-
Takagi Hiroshi
Department Of Bioscience Fukui Prefectural University
-
Takagi Hiroshi
Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University
-
Hasuura Yoshiyuki
Department Of Bioscience Fukui Prefectural University
関連論文
- Detection of ras Gene Mutations in Perioperative Peripheral Blood with Pancreatic Adenocarcinoma
- Immunohistochemical and Molecular Biological Studies of Serous Cystadenoma of the Pancreas
- Detection of Mutant K-ras in Dissected Paraaortic Lymph Nodes of Patients with Pancreatic Adenocarcinoma
- Neutrophil Behavior in Pancreas and Liver and the Role of Nitric Oxide in Rat Acute Pancreatitis
- Peritoneal Washing Cytology Combined with Immunocytochemical Staining and Detecting Mutant K-ras in Pancreatic Cancer : Comparison of the Sensitivity and Availability of Various Methods
- Detection of Hepatic Micrometastasis in Pancreatic Adenocarcinoma Patients by Two-stage Polymerase Chain Reaction/Restriction Fragment Length Polymorphism Analysis
- Temperature Dependence of the Yb 4f Spectra in Yb_4Bi_3 Probed by Photoemission Spectroscopy
- Factors Improving L-Threonine Production by a Three L-Threonine Biosynthetic Genes-amplified Recombinant Strain of Brevibacterium lactofermentum
- High Fermentative Production of L-Threonine from Acetate by a Brevibacterium flavum Stabilized Strain Transformed with a Recombinant Plasmid Carrying the Escherichia coli thr Operon
- Cloning and Characterization of Genes Responsible for m-Fluoro-D,L-phenylalanine Resistance in Brevibacterium lactofermentum(Microbiology & Fermentation Industry)
- Improvement of an L-Threonine Producer Derived from Brevibacterium flavum Using Threonine Operon of Escherichia coli K-12(Microbiology & Fermentation Industry)
- Bow Hunter's Stroke Associated with an Aberrant Course of the Vertebral Artery : Case Report
- Clinical Significance of Carcinoma Invasion of the Extrapancreatic Nerve Plexus in Pancreatic Cancer
- 2A09-4 Unique property of levodione reductase overexpressed in Escherichia coli cells : Inversion of stereospecificity in asymmetric reduction reaction
- Old Yellow Enzyme from Candida macedoniensis Catalyzes the Stereospecific Reduction of the C=C Bond of Ketoisophorone(Microbiology & Fermentation Technology)
- Occurrence of Multiple Ethyl 4-Chloro-3-Oxobutanoate-Reducing Enzymes in Candida magnoliae
- Cloning, Sequence Analysis, and Expression in Escherichia coli of the Gene Encoding Monovalent Cation-activated Levodione Reductase from Corynebacterium aquaticum M-13
- Experimental analysis of the fiber connection of the peptidergic systems in the central nervous system of the rat. I.
- Distribution of somatostatin in the rat central nervous system. I. Ontogenical aspects.
- Regional distribution of somatostatin in the avian central nervous system.
- Distribution of somatostatin in the fish brain (Carp)
- Distribution of somatostatin in the frog brain, Rana catesbiana.
- Morphological study on neuropeptides in the rat spinal cord (I)
- Ontogeny of the enkephalinergic neuron system in the rat lower brain stem
- Organization of some neuropeptides in the dorsal pontine tegmentum of the rat
- Ontogeny of the substance P neuron system in the rat lower brain stem
- Distribution of substance P in the frog brain, Rana catesbiana.
- Ontogeny of the substance P(SP) neuron system in the rat forebrain and upper brain stem.
- Distribution of substance P in the fish (Carp)
- Fine Structure of Noradrenaline Nerve Terminals in the N. Tractus Spinalis Nervi Trigemini of the Rats as demonstrated by a modified Potassium Permanganate Method
- Development of Expression Vectors for Thermus thermophilus
- Role of the DNA Sequence Downstream of the Bacillus subtilis hut Promoter in Regulation of the hut Operon
- Determination of the Transcriptional Initiation Sites of the Kanamycin Resistance Genes of pUB110 and pTHN1
- Prevention of Demyelination Induced by Rapid correction of Hyponatremia in Mice
- Purification and Properties of NAD-Linked 1,2-Butanediol Dehydrogenase from Rhodococcus sp. strain TB-42
- 12. Gene expressions of bone and cartilage matrix proteins during ectopic hard tissue formation induced by BMP.
- EFFECTS OF OPIOID PEPTIDES ON NEURONAL ACTIVITIES OF THE CNS : STUDIES USING MICROELECTROPHORETIC METHOD
- Thermoelectric Properties of Binary Semiconducting Intermetallic Compounds Al_2Ru and Ga_2Ru Synthesized by Spark Plasma Sintering Process
- First report of a 7-year survey on ABO-incompatible kidney transplantation in Japan
- Aderenocorticotropic Hormone (ACTH) Increases the Expression of Its Own Receptor Gene
- Unusual Transcription Regulation of the niaD Gene under Anaerobic Conditions Supporting Fungal Ammonia Fermentation
- Perpendicular Magnetic Anisotropy of TbCo Films
- Measurement of Magnetostriction of Amorphous Dy-Fe Films by Cantilever Capacitance Method
- Magnetostriction of Amorphous Gd-Fe and Tb-Fe Sputtered Films
- Partial Purification of Phytase from a Soil Isolate Bacterium, Klebsiella oxytoca MO-3
- Unusual Accessory Cranial Sutures in Pediatric Head Trauma
- Incidentally Discovered Adrenocortical Adenomas Are Not Fully Nonfunctioning: lmmunohistochemical and Dispersed Adrenocortical Cell Study
- Detection of Superoxide Free Radicals in Rats with Acute Pancreatitis
- LFA-1 (CD11a/CD18) and ICAM-1 (CD54) Antibodies Attenuate Superoxide Anion Release from Polymorphonuclear Leukocytes in Rats with Experimental Acute Pancreatitis
- The Involvement and Sources of Active Oxygen in Experimentally Induced Acute Pancreatitis
- Limited Proteolysis of Silkworm Antitrypsin by Several Proteinases(Biological Chemistry)
- The Entire Population of Thermus thermophilus Cells Is Always Competent at Any Growth Phase
- Overproduction of Carotenoids in Thermus thermophilus
- Isolation and Partial Characterization of Carotenoid Underproducing and Overproducing Mutants from an Extremetly Thermophilic Thermus thermophilus HB27
- Transcriptional Analysis of the ostA/imp Gene Involved in Organic Solvent Sensitivity in Escherichia coli
- The Production of Recombinant APRP, an Alkaline Protease Derived from Bacillus pumilus TYO-67, by In Vitro Refolding of Pro-enzyme Fixed on a Solid Surface
- Improved Autoprocessing Efficiency of Mutant Subtilisins E with Altered Specificity by Engineering of the Pro-Region^1
- Cryoprotective Effect of the Serine-Rich Repetitive Sequence in Silk Protein Sericin^1
- Secondary Hyperparathyroidism:Pathophysiology,Histopathology,and Medical and Surgical Management
- Purification and Characterization of 0-Acetylserine Sulfhydrylase of Corynebacterium glutamicum
- Cloning and Overexpression of the Exiguobacterium sp. F42 Gene Encoding a New Short Chain Dehydrogenase, Which Catalyzes the Stereoselective Reduction of Ethyl 3-Oxo-3-(2-thienyl)propanoate to Ethyl (S)-3-Hydroxy-3-(2-thienyl)propanoate
- Enhanced Thermostability of the Single-Cys Mutant Subtilisin E under Oxidizing Conditions^1
- 2S107 Metabolism and regulation of L-cysteine in Brevibacterium flavum (Corynebacterium glutamicum)
- POSSIBLE MECHANISM INVOLVED IN THE INHIBITORY ACTION OF U-50,488H, AN OPIOID κ AGONIST, ON GUINEA PIG HIPPOCAMPAL CA3 PYRAMIDAL NEURONS IN VITRO
- Fusion with Ribosomal Protein L32 Increased the in vivo Thermostability of Kanamycin Nucleotidyltransferase in Thermus thermophilus
- The α-Aminoadipate Pathway for Lysine Biosynthesis Is Widely Distributed among Thermus Strains
- Cloning and Sequence Analysis of Fumarase and Superoxide Dismutase Genes from an Extreme Thermophile Thermus thermophilus HB27
- Gait analysis in an osteoarthritis patient with restoration of the hip joint space following contralateral total hip arthroplasty : a case report
- TI-2 disc herniation following an en bloc cervical laminoplasty
- Image-Guided Anterior Thoracolumbar Corpectomy : A Report of Three Cases
- Phase II Trial of 5-Fluorouracil and Low-Dose Cisplatin in Patients with Squamous Cell Carcinoma of the Esophagus
- Tumor-specific Activation of Mitogen-activated Protein Kinase in Human Colorectal and Gastric Carcinoma Tissues
- Sional Transduction of GnRH Receptor in the Reproductive Tract Tumor
- Characterization of Novel Acetyltransferases Found in Budding and Fission Yeasts That Detoxify a Proline Analogue, Azetidine-2-Carboxylic Acid
- Development of Gene Delivery Systems for the ε-Poly-L-Lysine Producer, Streptomyces albulus(Genetics, Molecular Biology, and Gene Engineering)
- Overexpression and Characterization of an Aminoglycoside 6'-N-Acetyltransferase with Broad Specificity from an ε-Poly-L-lysine Producer, Streptomyces albulus IFO14147
- Cloning and Analysis of the β-Lactamase Gene from ε-Poly-L-lysine-Producing Actinomycete Streptomyces albulus IFO14147
- Effect of Proline and Arginine Metabolism on Freezing Stress of Saccharomyces cerevisiae(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Effect of Gene Disruption of Succinate Dehydrogenase on Succinate Production in a Sake Yeast Strain
- Efficient Selection for Thermostable Protease in Themus thermophilus
- Engineering Subtilisin E for Enhanced Stability and Activity in Polar Organic Solvents
- Isolation and Sequence Analysis of Plasmid pNO33 in the ε-Poly-_L-Lysine-Producing Actinomycete Streptomyces albulus IFO1417
- Effect of Gene Disruption of Succinate Dehydrogenase on Succinate Production in a Sake Yeast Strain
- Culture Conditions for Improvement of L-Threonine Production Using a Genetically Self-cloned L-Threonine Hyperproducing Strain of Escherichia coli K-12
- A Possible Vascular Malformation Origin for Perimesencephalic Nonaneurysmal Subarachnoid Hemorrhage
- Cerebral Bacterial Aneurysm and Indications for Cerebral Angiography in Infective Endocarditis
- Fenestration of the Intracranial Internal Carotid Artery : Case Report
- Neurinoma of the Oculomotor Nerve : Case Report
- Significance of Vascular Notching of Optic Nerves and Chiasm on Visual Disturbance
- S-I-13. U-Form External Decompression:Bilateral Fronto-Temporo-Parietal External Decompression
- B-49. Clinical Manifestatione and Operative Procedure of Upper Cervical Lesions-C_1・C_2 Fracture Dislocation and Atoanto-Axial Dislocation
- A-5. Clinical Manifestations and Operative Results of Frontal Head Injury
- HYDROLYTIC DEACTIVATION OF KYOTORPHIN BY THE RODENT BRAIN HOMOGENATES AND SERA
- Conversion of the Cleavage Specificity of Subtilisin YaB on Oxidized Insulin Chains to an Elastase-like Specificity by Replacement of Glyl24 with Ala(Biochemistry & Molecular Biology)
- A Novel Metal-Activated L-Serine O-Acetyltransferase from Thermus thermophilus HB8
- EFFECTS OF INTRAVENTRICULARLY ADMINISTERED SEROTONIN, NORADRENALINE, DOPAMINE AND METARAMINOL ON THE RESERPINE-INDUCED SPIKES RECORDED FROM THE MEDIAL NUCLEUS TRAPEZOIDES IN RABBITS
- EFFECTS OF SEVERAL MONOAMINE-RELATED COMPOUNDS ON THE RESERPINE-INDUCED SPIKES RECORDED FROM THE MEDIAL NUCLEUS TRAPEZOIDES IN RABBITS
- Plasmid Marker Rescue Transformation in Thermus thermophilus
- S-4. Atlanto-Axial Dislocation : Report of thirty cases
- Analysis of the Methionine Biosynthetic Pathway in the Extremely Thermophilic Eubacterium Thermus thermophilus