Development of Expression Vectors for Thermus thermophilus
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概要
- 論文の詳細を見る
We constructed expression vectors for Thermus thermophilus. The transformation efficiency of pTEV 131 per viable cells was 0.15% at 60℃ and 0.11% at 68℃, and with marker rescue transformation, it was 1.9% at 60℃ and 0.5% at 68℃. When the crtB gene was inserted into the cloning sites of this vector, the transformant expressed 3-fold more crtB gene than the wild type.
- 社団法人日本生物工学会の論文
- 1998-07-25
著者
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Hoshino Takayuki
Institute Of Applied Biochemistry University Of Tsukuba
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MASEDA HIDEAKI
Institute of Applied Biochemistry, University of Tsukuba
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Maseda Hideaki
Institute Of Applied Biochemistry University Of Tsukuba:(present Address)department Of Molecular Lif
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Maseda Hideaki
Institute Of Applied Biochemistry University Of Tsukuba
関連論文
- Development of Expression Vectors for Thermus thermophilus
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- Cloning and Sequence Analysis of Fumarase and Superoxide Dismutase Genes from an Extreme Thermophile Thermus thermophilus HB27
- Efficient Selection for Thermostable Protease in Themus thermophilus
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- Analysis of the Methionine Biosynthetic Pathway in the Extremely Thermophilic Eubacterium Thermus thermophilus