Isolation and Partial Characterization of Carotenoid Underproducing and Overproducing Mutants from an Extremetly Thermophilic Thermus thermophilus HB27

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概要

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• Twenty-two carotenoid underproducing and thirteen overproducing mutants were obtained from Thermus thermophilus HB27. The strain HB27 was found to produce at least seven colored carotenoids, believed to be identical to those produced by Thermus aquaticus YT1. Based on the results of the genetic analyses performed on twelve carotenoid underproducing mutants, they were classified into three groups; groups 1,2 and 3. No colored carotenoid was extracted from the cells of mutants belonging to groups 2 and 3,although the accumulation of phytoene, a colorless carotenoid, was observed in group 2 strains. Group 1 was subdivided into groups 1-a and 1-b, where 1-a strains produced neither colored carotenoids nor phytoene and 1-b strains produced two polar colored carotenoids. All of the overproducing mutants produced about twelve times as much seven colored carotenoid mixtures as the parental strain. The mutation loci among all the overproducing mutants were very close to one another, possibly in the same gene. Carotenoid overproducing mutants showed an extensive resistancy to UV-irradiation and showed poorer growth at higher temperatures. Carotenoid underproducing mutants were slightly more UV-sensitive but they grew almost normally at higher temperatures. These results suggest that carotenoids are secondary metabolites which are not essential for growth of T. thermophilus.

• 社団法人日本生物工学会の論文
• 1994-02-25

著者

• Hoshino T

  Institute Of Applied Biochemistry University Of Tsukuba Tsukuba City

• Hoshino Takayuki

  Institute Of Applied Biochemistry University Of Tsukuba

• Nakahara Tadaatsu

  Institute of Applied Biochemistry, University of Tsukuba

• Nakahara T

  Institute Of Applied Biochemistry University Of Tsukuba:(present Address)ryutsu Keizai University

• Nakahara Tadaatsu

  Institute Of Applied Biochemistry University Of Tsukuba

• FUJII RYOUJI

  Institute of Applied Biochemistry, University of Tsukuba.

• YOSHINO YUKO

  Institute of Applied Biochemistry, University of Tsukuba

• GUEVARRA ELVIRA

  Institute of Applied Biochemistry, University of Tsukuba

• ISHIDA SAYURI

  Institute of Applied Biochemistry, University of Tsukuba

• HIRUTA TOMOKO

  Institute of Applied Biochemistry, University of Tsukuba

• Yoshino Yuko

  Institute Of Applied Biochemistry University Of Tsukuba

• Fujii R

  Fukuyama Medical Lab. Co. Fukuyama Jpn

• Hiruta Tomoko

  Institute Of Applied Biochemistry University Of Tsukuba

• Ishida Sayuri

  Institute Of Applied Biochemistry University Of Tsukuba

• Guevarra Elvira

  Institute Of Applied Biochemistry University Of Tsukuba

• Nakahara Tadaatsu

  Institute Of Applied Biochemistry The University Of Tsukuba

• FUJII RYOUJI

  Institute of Applied Biochemistry, University of Tsukuba

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