Determination of the Polyester Polyurethane Breakdown Products and Distribution of the Polyurethane Degrading Enzyme of Comamonas acidovorans Strain TB-35

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概要

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• Metabolites produced by the degradation of polyester polyurethane (PUR) by Comamonas acidovorans strain TB-35 were investigated. GC-MS analysis revealed that they were diethylene glycol, trimethylolpropane and adipic aicd. These metabolites were considered to be derived from polyester segments of the PUR as a result of hydrolytic cleavage of ester bonds. In addition, when the culture broth was alkaline treated, a previously undetected product was detected by GC analysis. This product was identified as 2,4-diaminotoluene by GC-MS analysis. This indicates than an additional metabolite exists in the culture broth. This metabolite was considered to have been derived from polyisocyanate segments of the PUR. a preliminary study on a PUR degrading enzyme was also performed. Strain TB-35 produced two different esterases, one which is secreted to the culture broth and one which is bound to the cell surface. Between them, only cell-surface-bound esterase catalyzes the degradation of the polyester PUR.

• 社団法人日本生物工学会の論文
• 1997-05-25

著者

• Nakajima-kambe Toshiaki

  Institute Of Applied Biochemistry University Of Tsukuba

• Nakahara Tadaatsu

  Institute of Applied Biochemistry, University of Tsukuba

• ONUMA FUMIKO

  Institute of Applied Biochemistry, University of Tsukuba

• AKUTSU YUKIE

  Institute of Applied Biochemistry, University of Tsukuba

• Nakahara Tadaatsu

  Institute Of Applied Biochemistry University Of Tsukuba

• Akutsu Yukie

  Institute Of Applied Biochemistry University Of Tsukuba

• Onuma Fumiko

  Institute Of Applied Biochemistry University Of Tsukuba

• Nakahara Tadaatsu

  Institute Of Applied Biochemistry The University Of Tsukuba

• Nakajima-Kambe Toshiaki

  Institute of Applied Biochemistry, University of Tsukuba

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