2-Methylcitrate Dehydratase, a New Enzyme Functioning at the Methylcitric Acid Cycle of Propionate Metabolism
スポンサーリンク
概要
- 論文の詳細を見る
2-Methylcitrate dehydratase (2-methylcitrate hydro-lyase), a new enzyme functioning at the methylcitric acid cycle of propionyl-CoA oxidation, was present in the cell-free extract of Yarrowia (Saccharomycopsis) lipolytica. The enzyme was separated from the usual aconitate hydratase (EC 4.2.1.3) of the yeast with DEAE-Sephadex A-50 column chromatography. The enzyme was able to catalyze a reversible reaction between 2-methylcitrate and 2-methyl-cis-aconitate, but showed no activity on threo-Ds-2-methylisocitrate, citrate, cis- or trans-aconitate, threo-Ds-, threo-DL- or erythro-Ls-isocitrate, DL-homocitrate or other hydroxy-acids tested. In contrast, the other enzyme fraction separated as aconitate hydratase by chromatography showed no activity on synthetic 2-methylcitrate, but was able to catalyze strongly a reversible reaction between 2-methyl-cis-aconitate and threo-Ds-2-methylisocitrate. From these findings, the previously proposed cycle sequence was revised at the following broken arrows: propionyl-CoA+oxaloacetate → (CoASH+) 2-methylcitrate → 2-methyl-cis-aconitate → threo-Ds-2-methylisocitrate → pyruvate + succinate (→→ oxaloacetate). 2-Methylcitrate dehydratase showed maximum activity at pH 6.5 to 7.0 and at 25 to 40°C. The enzyme was stable at temperatures up to 40°C and at pH 6.5 to 7.5, but labile in Tris-HCl buffer. The synthesis of this enzyme was constitutive in this yeast, although it was slightly repressed by glucose.
- 社団法人 日本農芸化学会の論文
著者
-
Tabuchi Takeshi
Institute Of Applied Biochemistry The University Of Tsukuba
-
Aoki Hiroyuki
Institute of Applied Biochemistry, University of Tsukuba
-
Nakahara Tadaatsu
Institute Of Applied Biochemistry The University Of Tsukuba
-
UCHIYAMA Hiroo
Institute of Applied Biochemistry, The University of Tsukuba
関連論文
- Visualizing the Effects of Biofilm Structures on the Influx of Fluorescent Material Using Combined Confocal Reflection and Fluorescent Microscopy
- Purification and Properties of NAD-Linked 1,2-Butanediol Dehydrogenase from Rhodococcus sp. strain TB-42
- Production of 2-Hydroxybutyric Acid from 1,2-Butanediol by Resting Cells of Rhodococcus sp. Strain TB-42
- PB-38 Identification of methane monooxygenase operons from metagenome by combining stable isotope probing(Genetic analysis,Poster presentation B)
- Matrix Metalloproteinase-2 Inhibition and Zn^-Chelating Activities of Pyoverdine-Type Siderophores (MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Participation of Nitrite Reductase in Conversion of NO_2^- to NO_3^- in a Heterotrophic Nitrifier, Burkholderia cepacia NH-17, with Denitrification Activity
- Cloning and Sequence Analysis of Poly(tetramethylene succinate) Depolymerase from Acidovorax delafieldii Strain BS-3
- Purification and Properties of Culture-Broth-Secreted Esterase from the Polyurethane Degrader Comamonas acidovorans TB-35
- Biodegradation of Dibenzothiophene and 4, 6-Dimethyldiben-zothiophene by Sphingomonas paucimobilis Strain TZS-7
- Decolorization of Molasses Wastewater by Bacillus sp.under Thermophilic and Anaerobic Conditions
- Cloning and Sequence Analysis of a Polyurethane Esterase of Comamonas acidovorans TB-35
- Efficient Conversion of Itaconic Acid to (S)-(+)-Citramalic Acid by Alcaligenes xylosoxydans IL142
- Bioconversion of Maleic Acid to Fumaric Acid by Pseudomonas alcaligenes Strain XD-1
- Production of (S)-(+)-3-Hydroxybutyric Acid from 1,3-Butanediol by Resting Cells of Yeasts
- Determination of the Polyester Polyurethane Breakdown Products and Distribution of the Polyurethane Degrading Enzyme of Comamonas acidovorans Strain TB-35
- The Response of Pseudomonas aeruginosa PAO1 Efflux Pump-Defective Mutants to N-Octanoyl-L-Homoserine Lactone
- Production of Mannosylerythritol Lipids by Candida antarctica from Vegetable Oils(Microbiology & Fermentation Industry)
- Extracellular Accumulation of Mannosylerythritol Lipids by a Strain of Candida antarctica(Microbiology & Fermentation Industry)
- Analysis of Dibenzothiophene Metabolic Pathway in Mycobacterium Strain G3
- Analyses of Substrate Specificity of the Desulfurizing Bacterium Mycobacterium sp. G3
- Poly(β-Malic Acid) Production by the Non-Growing Cells of Aureobasidium sp. Strain A-91
- A Quorum-Sensing Autoinducer Enhances the mexAB-oprM Efflux-Pump Expression without the MexR-Mediated Regulation in Pseudomonas aeruginosa
- Purification and Characterization of an Intracellular α-D-Xylosidase from Penicillium wortmannii IFO 7237
- Functions and Potential Applications of Glycolipid Biosurfactants : from Energy-Saving Materials to Gene Delivery Carriers
- Microbial Extracellular Glycolipid Induction of Differentiation and Inhibition of the Protein Kinase C Activity of Human Promyelocytic Leukemia Cell Line HL60
- The Entire Population of Thermus thermophilus Cells Is Always Competent at Any Growth Phase
- Overproduction of Carotenoids in Thermus thermophilus
- Isolation and Partial Characterization of Carotenoid Underproducing and Overproducing Mutants from an Extremetly Thermophilic Thermus thermophilus HB27
- Induction and Characterization of Mutants Enhanced in Assimilability of n-Alkanes in Shake Cultures from a Strain of Candida sp.
- Itaconic Acid Fermentation by a Yeast Belonging to the Genus Candida
- Factors Affecting the Production of Succinoyl Trehalose Lipids by Rhodococcus erythropolis SD-74 Grown on n-Alkanes(Microbiology & Fermentation Industry)
- Extracellular Accumulation of Mono- and Di-Succinoyl Trehalose Lipids by a Strain of Rhodococcus erythropolis Grown on n-Alkanes(Microbiology & Fermentation Industry)
- Production of D-Malic Acid from Maleic Acid by Resting Cells of Ustilago sphaerogena Strain S402
- The Effects of Culture Conditions on the Secretion of Human Lysozyme by Saccharomyces cerevisiae A2-1-1A(Biological Chemistry)
- Fermentative Production of Poly(β-L-malic acid), a Polyelectrolytic Biopolyester, by Aureobasidium sp.
- Characteristics of 2-Methylisocitrate Dehydratase, Isolated from Yallowia lipolytica, in Comparison with Aconitase
- Isolation of 2-Methylisocitrate Dehydratase, a New Enzyme Serving in the Methylcitric Acid Cycle for Propionate Metabolism, from Yallowia lipolytica
- Aerobic Degradation of Trichloroethylene by a New Type II Methane-Utilizing Bacterium, Strain M(Microbiology & Fermentation Industry)
- Aerobic Degradation of Trichloroethylene at High Concentration by a Methane-Utilizing Mixed Culture(Microbiology & Fermentation Industry)
- Extracellular Accumulation of the Polyol Lipids, 3,5-Dihydroxydecanoyl and 5-Hydroxy-2-decenoyl Esters of Arabitol and Mannitol, by Aureobasidium sp
- Role of Methylcitric Acid Cycle in Catabolism of Amino Acids by Saccharomycopsis lipolytica(Microbiology & Fermentation Industry)
- Antiproliferative Effect of Polyol Lipids, 3,5-Dihydroxydecanoyl and 5-Hydroxy-2-Decenoyl Esters of Arabitol and Mannitol on Lung Cancer Cell Line A549
- 413 β-Hydroxyparaconic and Itatartaric Acid Fermentation by Ustilago cynodontis and Simple Recovery Methods for the Acids
- Accumulation of Itaconic, 2-Hydroxyparaconic, Itatartaric, and Malic Acids by Strains of the Genus Ustilago(Microbiology & Fermentation Industry)
- Effect if Hydrocarbon Emulsification on Growth and Respiration of Microorganisms in Hydrocarbon Media
- Difference in Degrading p-Nitrophenol between Indigenous Bacteria in a Reactor
- Extracellular Production of a Mannosylerythritol Lipid by a Mutant of Candida sp. from n-Alkanes and Triacylglycerols
- Production of Yeast Cells from Palm Oil at the High Temperature of 40℃
- Production of 2-Hydroxyparaconic and Itatartaric Acids by Ustilago cynodontis and Simple Recovery Process of the Acids(Microbiology & Fermentation Industry)
- Factors Affecting the Production of the Open-ring Acid of Spiculisporic Acid by Penicillium spiculisporum
- Accumulation of the Open-ring Acid of Spiculisporic Acid by Penicillium spiculisporum in Shake Culture
- Extracellular Production of D-(+)-2-Hydroxyglutaric Acid by Yarrowia lipolytica from Glucose under Aerobic, Thiamine-deficient Conditions
- Isocitric Acid Production from n-Alkanes by Candida catenulata
- Production of Isocitric Acid from Glucose by Candida ravautii
- A New n-Alkane Oxidation System from Pseudomonas aeruginosa S7B1
- Fermentative Production of 3-Hydroxy-3-methylglutaric Acid by Candida sorbosa
- Purification and Properties of 2-Methylcitrate Dehydratase from Yarrowia lipolytica
- 2-Methylcitrate Dehydratase, a New Enzyme Functioning at the Methylcitric Acid Cycle of Propionate Metabolism
- Distribution of the Methylcitric Acid Cycle and β-Oxidation Pathway for Propionate Catabolism in Fungi