Purification and Properties of NAD-Linked 1,2-Butanediol Dehydrogenase from Rhodococcus sp. strain TB-42

スポンサーリンク

概要

• 論文の詳細を見る

• 1, 2-Butanediol (1, 2-BD) dehydrogenase was purified from Rhodococcus sp. strain TB-42. The activity of this enzyme was induced by 1, 2-BD and the highest activity was observed in the cells grown on 1, 2-BD as a carbon source. The native enzyme had a molecular mass of 80 kDa, and was composed of two different subunits with molecular masses of 46kDa and 43kDa. The enzyme showed a pH optimum of 10.5, and displayed the highest activity at 35-40℃. NADP could not replace NAD as an electron acceptor for the reaction. The enzyme oxidized diols with two adjacent hydroxy groups such as 1, 2-propanediol, 1, 2-BD, 2, 3-BD, and 1, 2-pentanediol except for 1, 2-ethanediol. Other diols, primary and secondary alcohols, could not be used as substrates for this enzyme.

• 社団法人日本農芸化学会の論文
• 1995-02-23

著者

• SATO Shinji

  Institute of Engineering Mechanics, University of Tsukuba

• Nakajima Toshiaki

  Institute Of Applied Biochemistry University Of Tsukuba

• Nakajima-kambe Toshiaki

  Institute Of Applied Biochemistry University Of Tsukuba

• Hoshino T

  Institute Of Applied Biochemistry University Of Tsukuba

• Hoshino Takayuki

  Institute Of Applied Biochemistry University Of Tsukuba

• Sawai Satsuki

  Institute of Applied Biochemistry, University of Tsukuba

• Nakahara Tadaatsu

  Institute of Applied Biochemistry, University of Tsukuba

• Nakajima-kambe Toshiaki

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Serada S

  Department Of Applied Biology Kyoto Institute Of Technology

• Nakajima T

  Suntory Institute For Bioorganic Research

• Sato S

  From The Department Of Applied Biology Kyoto Institute Of Technology

• Nakahara T

  Institute Of Applied Biochemistry University Of Tsukuba:(present Address)ryutsu Keizai University

• Nakahara Tadaatsu

  Graduate School Of Life And Environmental Science University Of Tsukuba

• Nakahara Tadaatsu

  Institute Of Applied Biochemistry University Of Tsukuba

• Sawai Satsuki

  Institute Of Applied Biochemistry University Of Tsukuba

• Nakae T

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Sato Syunichi

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• Nakahara Tadaatsu

  Institute Of Applied Biochemistry The University Of Tsukuba

• Sato Shinji

  Institute for Materials Research, Tohoku University

関連論文

• The IRTS(Infrared Telescope in Space)Mission
• Development of Expression Vectors for Thermus thermophilus
• Role of the DNA Sequence Downstream of the Bacillus subtilis hut Promoter in Regulation of the hut Operon
• Determination of the Transcriptional Initiation Sites of the Kanamycin Resistance Genes of pUB110 and pTHN1
• PB-07 異種微生物間コミュニケーションによるPseudomonas aeruginosaの抗生物質耐性、病原性、遺伝子発現制御の発見(共生/相互作用,ポスターセッションB,(1)ポスター発表会,研究発表会)
• Purification and Properties of NAD-Linked 1,2-Butanediol Dehydrogenase from Rhodococcus sp. strain TB-42
• Production of 2-Hydroxybutyric Acid from 1,2-Butanediol by Resting Cells of Rhodococcus sp. Strain TB-42
• High Catalase Production by Rhizobium radiobacter Strain 2-1(ENVIRONMENTAL BIOTECHNOLOGY)
• PB-85 Involvement of a respiratory terminal oxidase in Pseduomonas aeruginosa biofilm formation(Surface/Biofilm,Poster presentation B)
• PB-84 Studies on Natural Transformation of Pseudomonas aeruginosa(Surface/Biofilm,Poster presentation B)
• PB-38 Identification of methane monooxygenase operons from metagenome by combining stable isotope probing(Genetic analysis,Poster presentation B)
• Rapid monomerization of poly(butylene succinate)-co-(butylene adipate) by Leptothrix sp.(ENVIRONMENTAL BIOTECHNOLOGY)
• Pseudomonas Quinolone Signal Affects Membrane Vesicle Production in not only Gram-Negative but also Gram-Positive Bacteria
• Matrix Metalloproteinase-2 Inhibition and Zn^-Chelating Activities of Pyoverdine-Type Siderophores (MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Participation of Nitrite Reductase in Conversion of NO_2^- to NO_3^- in a Heterotrophic Nitrifier, Burkholderia cepacia NH-17, with Denitrification Activity
• A Simple Screening Procedure for Heterotrophic Nitrifying Bacteria with Oxygen-Tolerant Denitrification Activity
• Cloning and Sequence Analysis of Poly(tetramethylene succinate) Depolymerase from Acidovorax delafieldii Strain BS-3
• 145 Cloning and expression of a poly (lactic acid) and poly (butylene succinate-co-adipate) depolymerase
• Purification and Properties of Culture-Broth-Secreted Esterase from the Polyurethane Degrader Comamonas acidovorans TB-35
• Biodegradation of Dibenzothiophene and 4, 6-Dimethyldiben-zothiophene by Sphingomonas paucimobilis Strain TZS-7
• Decolorization of Molasses Wastewater by Bacillus sp.under Thermophilic and Anaerobic Conditions
• Cloning and Sequence Analysis of a Polyurethane Esterase of Comamonas acidovorans TB-35
• Efficient Conversion of Itaconic Acid to (S)-(+)-Citramalic Acid by Alcaligenes xylosoxydans IL142
• Bioconversion of Maleic Acid to Fumaric Acid by Pseudomonas alcaligenes Strain XD-1
• The Effect of a Cell-to-Cell Communication Molecule, Pseudomonas Quinolone Signal (PQS), Produced by P. aeruginosa on Other Bacterial Species
• Production of (S)-(+)-3-Hydroxybutyric Acid from 1,3-Butanediol by Resting Cells of Yeasts
• Determination of the Polyester Polyurethane Breakdown Products and Distribution of the Polyurethane Degrading Enzyme of Comamonas acidovorans Strain TB-35
• Production of Mannosylerythritol Lipids by Candida antarctica from Vegetable Oils(Microbiology & Fermentation Industry)
• Identification and Functional Analysis of Genes Required for Desulfurization of Alkyl Dibenzothiophenes of Mycobacterium sp. G3(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Analysis of Dibenzothiophene Metabolic Pathway in Mycobacterium Strain G3
• Analyses of Substrate Specificity of the Desulfurizing Bacterium Mycobacterium sp. G3
• Poly(β-Malic Acid) Production by the Non-Growing Cells of Aureobasidium sp. Strain A-91
• Characterization of the Aerobic Denitrification in Mesorhizobium sp. Strain NH-14 in Comparison with that in Related Rhizobia
• Screening of Novel Cellulose-Degrading Bacterium and Its Application to Denitrification of Groundwater(Environmental Biotechnology)
• Insulin/IGF-like Peptides of the Nematode Caenorhabditis elegans : Structures and Analyses of the Expression Pattern and Physiological Function
• A New Polypeptide Toxin from the Nematocyst Venom of an Okinawan Sea Anemone Phyllodiscus semoni (Japanese name "unbachi-isoginchaku")(Biochemistry & Molecular Biology)
• A Novel Protein Toxin from the Deadly Box Jellyfish (Sea Wasp, Habu-kurage) Chiropsalmus quadrigatus(Biochemistry & Molecular Biology)
• Unusual Transcription Regulation of the niaD Gene under Anaerobic Conditions Supporting Fungal Ammonia Fermentation
• Purification and Characterization of an Intracellular α-D-Xylosidase from Penicillium wortmannii IFO 7237
• Functions and Potential Applications of Glycolipid Biosurfactants : from Energy-Saving Materials to Gene Delivery Carriers
• Microbial Extracellular Glycolipid Induction of Differentiation and Inhibition of the Protein Kinase C Activity of Human Promyelocytic Leukemia Cell Line HL60
• The Entire Population of Thermus thermophilus Cells Is Always Competent at Any Growth Phase
• Overproduction of Carotenoids in Thermus thermophilus
• Isolation and Partial Characterization of Carotenoid Underproducing and Overproducing Mutants from an Extremetly Thermophilic Thermus thermophilus HB27
• Cloning and Characterization of a Caenorhabditis elegans cDNA Encoding a New Insulin/IGF-Like Peptide(Biochemistry & Molecular Biology)
• Induction and Characterization of Mutants Enhanced in Assimilability of n-Alkanes in Shake Cultures from a Strain of Candida sp.
• Itaconic Acid Fermentation by a Yeast Belonging to the Genus Candida
• Neutralization of Toxic Heme by Plasmodium Falciparum Histidine-Rich Protein 2
• Effect of Antifungal Azoles on the Heme Detoxification System of Malarial Parasite
• Factors Affecting the Production of Succinoyl Trehalose Lipids by Rhodococcus erythropolis SD-74 Grown on n-Alkanes(Microbiology & Fermentation Industry)
• Production of D-Malic Acid from Maleic Acid by Resting Cells of Ustilago sphaerogena Strain S402
• Purification and Properties of a Soluble Methane Monooxygenase from Methylocystis sp. M
• Novel Metabolite of Trichloroethylene in a Methanotrophic Bacterium, Methylocystis sp. M, and Hypothetical Degradation Pathway
• The Effects of Culture Conditions on the Secretion of Human Lysozyme by Saccharomyces cerevisiae A2-1-1A(Biological Chemistry)
• Fermentative Production of Poly(β-L-malic acid), a Polyelectrolytic Biopolyester, by Aureobasidium sp.
• Fusion with Ribosomal Protein L32 Increased the in vivo Thermostability of Kanamycin Nucleotidyltransferase in Thermus thermophilus
• The α-Aminoadipate Pathway for Lysine Biosynthesis Is Widely Distributed among Thermus Strains
• Cloning and Sequence Analysis of Fumarase and Superoxide Dismutase Genes from an Extreme Thermophile Thermus thermophilus HB27
• Cloning and characterization of a Drosophila melanogaster cDNA encoding a glutamate transporter
• Structures of Insulin-like Peptides of the Nematode Caenorhabditis elegans
• Structure and Activity of a New Form of the Glutamate Transporter of the Nematode Caenorhabditis elegans
• Molecular Cloning of a cDNA Derived from The Nematode Caenorhabditis Elegans Encoding The Peptide Promoting Uptake of Glutamate
• Aerobic Degradation of Trichloroethylene by a New Type II Methane-Utilizing Bacterium, Strain M(Microbiology & Fermentation Industry)
• Aerobic Degradation of Trichloroethylene at High Concentration by a Methane-Utilizing Mixed Culture(Microbiology & Fermentation Industry)
• Efficient Selection for Thermostable Protease in Themus thermophilus
• Extracellular Accumulation of the Polyol Lipids, 3,5-Dihydroxydecanoyl and 5-Hydroxy-2-decenoyl Esters of Arabitol and Mannitol, by Aureobasidium sp
• Study on the Application of the (S)-2-tert-Butyl-2-methyl-1,3-benzodioxole-4-carboxylic acid-based On-line HPLC-Exciton CD Analysis to Acyclic 1,3-Diols
• Plasmid Marker Rescue Transformation in Thermus thermophilus
• Assimilative and co-metabolic degradation of chloral hydrate by bacteria and their bioremediation potential
• Diversity and Similarity of Microbial Communities in Petroleum Crude Oils Produced in Asia
• Antiproliferative Effect of Polyol Lipids, 3,5-Dihydroxydecanoyl and 5-Hydroxy-2-Decenoyl Esters of Arabitol and Mannitol on Lung Cancer Cell Line A549
• Effect if Hydrocarbon Emulsification on Growth and Respiration of Microorganisms in Hydrocarbon Media
• Difference in Degrading p-Nitrophenol between Indigenous Bacteria in a Reactor
• Extracellular Production of a Mannosylerythritol Lipid by a Mutant of Candida sp. from n-Alkanes and Triacylglycerols
• Production of Yeast Cells from Palm Oil at the High Temperature of 40℃
• Study on the Application of the (S)-2-tert-Butyl-2-methyl-1,3-benzodioxole-4-carboxylic acid-based On-line HPLC-Exciton CD Analysis to Acyclic Vicinal Diols and 1,3-Diols
• Analysis of the Methionine Biosynthetic Pathway in the Extremely Thermophilic Eubacterium Thermus thermophilus
• Pseudomonas Quinolone Signal Affects Membrane Vesicle Production in not only Gram-Negative but also Gram-Positive Bacteria
• Rapid and simple colorimetric assay for detecting the enzymatic degradation of biodegradable plastic films(METHODS)
• Extracellular Production of D-(+)-2-Hydroxyglutaric Acid by Yarrowia lipolytica from Glucose under Aerobic, Thiamine-deficient Conditions
• Isocitric Acid Production from n-Alkanes by Candida catenulata
• Production of Isocitric Acid from Glucose by Candida ravautii
• A New n-Alkane Oxidation System from Pseudomonas aeruginosa S7B1
• Fermentative Production of 3-Hydroxy-3-methylglutaric Acid by Candida sorbosa
• 2-Methylcitrate Dehydratase, a New Enzyme Functioning at the Methylcitric Acid Cycle of Propionate Metabolism

もっと見る 閉じる

スポンサーリンク