A Novel Protein Toxin from the Deadly Box Jellyfish (Sea Wasp, Habu-kurage) Chiropsalmus quadrigatus(Biochemistry & Molecular Biology)
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概要
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The deadly box jellyfish (Sea Wasp, Habu-kurage in Japanese) Chiropsalmus quadrigatus Haeckel (Cubozoa) is distributed widely in the tropical Pacific region. In Japan, three fatal cases due to stings from this species have been reported officially. We successfully isolated C. quadrigatus toxin-A (CqTX-A, 44 kDa), a major proteinaceous toxin, for the first time, from the nematocysts of C. quadrigatus. CqTX-A showed lethal toxicity to crayfish when administered via intraperitoneal injection (LD_<50> = 80μg/kg) and hemolytic activity toward 0.8% sheep red blood cells (ED_<50> = 160 ng/ml). Furthermore, we sequenced the cDNA encoding CqTX-A. The deduced amino acid sequence of CqTX-A (462 amino acids) showed 25.2% and 21.6% sequence similarity to Carybdea rastoni toxins (CrTXs) and Carybdea alata toxin-A (CrTX-A), respectively, which are Cubozoan jellyfish toxins.
- 社団法人日本農芸化学会の論文
- 2002-01-23
著者
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Takuwa-Kuroda Kyoko
SUNTORY INSTITUTE FOR BIOORGANIC RESEARCH
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Oshiro N
Okinawa Prefectural Inst. Health And Environment Okinawa Jpn
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NAKAJIMA Terumi
Suntory Institute for Bioorganic Research
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Iwanaga Setsuko
Okinawa Prefectural Institute Of Health And Environment
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Iwanaga Setsuko
Okinawa Prefectural Agricultural Experiment Station
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Oshiro Naomasa
Okinawa Prefecture Istitute Of Health And Environment
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NAGAI Hiroshi
Suntory Institute for Bioorganic Research
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NAKAO Masahiro
Institute for Biomedical Research, Suntory Ltd.
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Oshiro N
Laboratories Of Applied Biochemistry University Of The Ryukyus
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Nakajima T
Suntory Institute For Bioorganic Research
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Nagai Hiroshi
Department Of Ocean Sciences Tokyo University Of Marine Science And Technology
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Nagai H
Suntory Institute For Bioorganic Research:(present Address)tokyo University Of Fisheries
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Nakao M
Institute For Biomedical Research Suntory Ltd.
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Iwanaga Shiroh
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Nagai H
Department Of Ocean Sciences Tokyo University Of Marine Science And Technology
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Nakao Masahiro
Institute For Biomedical Research Suntory Ltd.
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NAKAO Masahiro
Institute for Advanced Technology, Technological Development Center, Suntory Ltd.
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