Cloning and Sequence Analysis of Poly(tetramethylene succinate) Depolymerase from Acidovorax delafieldii Strain BS-3

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概要

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• A gene encoding poly(tetramethylene succinate), PBS, depolymerase, pbsA, has been cloned from Acidovorax delafieldii strain BS-3 chromosomal DNA. The clone expressed in Escherichia coli showed the ability to degrade both PBS and poly{(tetramethylene succinate)-co-adipate} that are kinds of biodegradable plastics. PBS depolymerase was considered to be a kind of lipase, since it also degrades olive oil. It had no apparent hydrophobic-amino-acid-rich region which exists in other known plastic-degrading enzymes. From the result of amino acid homology search, PbsA was found to have some similarities with lipases of Streptomyces sp. and Mollaxella sp. In the motif surrounding the active site Ser residue (Gly-X1-Ser-X2-Gly), PbsA was revealed to have a Trp residue in the X1 position instead of His which is most likely found in other bacterial lipases.

• 社団法人日本生物工学会の論文
• 2002-02-25

著者

• UCHIDA HIROMI

  Institute of Applied Biochemistry, University of Tsukuba

• UCHIYAMA HIROO

  Institute of Life and Environmental Sciences, University of Tsukuba

• NOMURA NOBUHIKO

  Institute of Life and Environmental Sciences, University of Tsukuba

• Nakajima Toshiaki

  Institute Of Applied Biochemistry University Of Tsukuba

• Nakajima-kambe Toshiaki

  Institute Of Applied Biochemistry University Of Tsukuba

• Uchiyama Hiroo

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Uchiyama H

  National Inst. Environmental Studies Ibaraki Jpn

• Uchiyama H

  National Institute For Environmental Studies

• Uchiyama Hiroo

  National Institute Of Environmental Studies

• Uchiyama Hiroo

  Water And Soil Environment Division National Institute For Enviromental Studies

• Nomura Nobuhiko

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Nomura Nobuhiko

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Nomura Nobuhiko

  Institute Of Applied Biochemistry University Of Tsukuba

• Nakahara Tadaatsu

  Institute of Applied Biochemistry, University of Tsukuba

• SHIGENO-AKUTSU YUKIE

  Institute of Applied Biochemistry, University of Tsukuba

• Nakajima-kambe Toshiaki

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Nakahara Tadaatsu

  Graduate School Of Life And Environmental Science University Of Tsukuba

• Nakahara Tadaatsu

  Institute Of Applied Biochemistry University Of Tsukuba

• Shigeno-akutsu Yukie

  Institute Of Applied Biochemistry University Of Tsukuba

• Nomura Nobuhiko

  Institute Of Life And Environmental Sciences University Of Tsukuba

• Nakae T

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Nomura N

  Institute Of Applied Biochemistry University Of Tsukuba

• Nakahara Tadaatsu

  Institute Of Applied Biochemistry The University Of Tsukuba

• Uchida Hiromi

  Institute Of Applied Biochemistry University Of Tsukuba

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