Analyses of Substrate Specificity of the Desulfurizing Bacterium Mycobacterium sp. G3

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概要

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• The substrate specificity of Mycobacterium sp. G3 with desulfurization activity against dibenzothiophene (DBT) was investigated. Desulfurization reactions were carried out using a concentrated cell suspension of G3. The conversion from 4,6-dipropyl DBT, one of the sulfur-containing compounds that is difficult to desulfurize in diesel oil, to 2-hydroxy-3,3'-dipropylbiphenyl as an end -product of the desulfurization reaction was found in the water reaction system and in the oil/water two-phase reaction system. 4,6-Dibutyl DBT and 4,6-dipentyl DBT were metabolized to the hydroxybiphenyl form via the sulfone form in the water reaction system. These results indicate that G3 has high membrane permeability and superior substrate specificity for high molecular weight alkyl DBTs, which are represented by 4,6-dipentyl DBT as C10-DBT. Furthermore, G3 could desulfurize diesel oil, and the sulfur concentration was decreased from 116 mg l^<-1> to 48 mg l^<-1> within 24 h.

• 社団法人日本生物工学会の論文
• 2002-02-25

著者

• NOMURA NOBUHIKO

  Institute of Life and Environmental Sciences, University of Tsukuba

• MARUHASHI KENJI

  Bio-Refining Process Laboratory, Japan Cooperation Center, Petroleum

• Nomura Nobuhiko

  Institute Of Applied Biochemistry University Of Tsukuba

• Nakahara Tadaatsu

  Institute of Applied Biochemistry, University of Tsukuba

• Okada Hideki

  Research And Development Center Cosmo Oil Co. Ltd.

• OKADA HIDEKI

  Bio-Refining Process Laboratory, Technical Cooperation Department, Japan Cooperation Center, Petrole

• Nakajima-kambe Toshiaki

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Okada Hideki

  Shin Nihon Chemical Co. Ltd

• Nakahara T

  Institute Of Applied Biochemistry University Of Tsukuba:(present Address)ryutsu Keizai University

• Nakahara Tadaatsu

  Institute Of Applied Biochemistry University Of Tsukuba

• Nomura Nobuhiko

  Institute Of Life And Environmental Sciences University Of Tsukuba

• Maruhashi Kenji

  Bio-refining Process Laboratory Technical Cooperation Department Japan Cooperation Center

• Nomura N

  Institute Of Applied Biochemistry University Of Tsukuba

• Nakahara Tadaatsu

  Institute Of Applied Biochemistry The University Of Tsukuba

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