Improvement of Desulfurization Activity in Rhodococcus erythropolis KA2-5-1 by Genetic Engineering

スポンサーリンク

概要

• 論文の詳細を見る

• Rhodococcus erythropolis KA2-5-1 can desulfurize dibenzothiophene (DBT) into 2-hydroxybiphenyl. A cryptic plasmid, pRC4,which was derived from R.rhodochrous IFO3338,was combined with an Escherichia coli vector to construct an E.coli-Rhodococcus shuttle vector. The complete nucleotide sequence of 2582-bp pRC4 was analyzed. Based on the characteristics of its putative replication genes, pRC4 was assigned to the family of pAL5000-related replicons. The desulfurization gene cluster, dszABC, and the related reductase gene, dszD, cloned from KA2-5-1,were reintroduced into KA2-5-1 and efficiently expressed. The DBT desulfurization ability of the transformant carrying two dszABC clusters and one dszD on the vector was about 4-fold higher than that of the parent strain, and the transformant also showed improved desulfurization activity for light gas oil (LGO). Sulfur components in LGO before and after the reaction were analyzed with gas chromatography-atomic emission detection.

• 社団法人日本農芸化学会の論文
• 2001-02-23

著者

• Kobayashi Miki

  Biochemicals Laboratory Mitsubishi Chemical Corporation

• Izumi Yoshikazu

  Dep. Of Biotechnology Tottori Univ.

• Ishii Yoshitaka

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• ISHII YOSHITAKA

  Bio-Refining Process Laboratory, Japan Cooperation Center, Petroleum

• MARUHASHI KENJI

  Bio-Refining Process Laboratory, Japan Cooperation Center, Petroleum

• Ishii Yoshitaka

  Institute For Biomedical Engineering Consolidated Research Institute For Advanced Science And Medica

• Kobayashi M

  Carbohydrate Science Laboratory National Food Research Institute Ministry Of Agriculture Forestry An

• KOBAYASHI Morio

  Bio-Refining Process Laboratory, Advanced Technology and Research Institute, Petroleum Energy Center

• HIRASAWA Kazuaki

  Bio-Refining Process Laboratory, Advanced Technology and Research Institute, Petroleum Energy Center

• KOIZUMI Kenichi

  Bio-Refining Process Laboratory, Advanced Technology and Research Institute, Petroleum Energy Center

• Maruhashi Kenji

  Bio-refining Process Laboratory Technical Cooperation Department Japan Cooperation Center

• Kobayashi Morio

  Bio-refining Process Laboratory Advanced Technology And Research Institute Petroleum Energy Center

• Koizumi Kenichi

  Bio-refining Process Laboratory Advanced Technology And Research Institute Petroleum Energy Center

• Ishii Yoshitaka

  Bio-refining Process Laboratory Advanced Technology And Research Institute Petroleum Energy Center

• Hirasawa Kazuaki

  Bio-refining Process Laboratory Advanced Technology And Research Institute Petroleum Energy Center

関連論文

• Characterization and Distribution of IS1164 that Exists in the High Molecular Mass Nitrile Hydratase Gene Cluster of the Industrial Microbe Rhodococcus rhodochrous J1
• Characterization of the Second Form of NADH-Cytochrome b_5 Reductase Gene from Arachidonic Acid-Producing Fungus Mortierella alpina 1S-4
• Genetic Analysis of Cytochrome b_5 from Arachidonic Acid-Producing Fungus, Mortierella alpina 1S-4 : Cloning, RNA Editing and Expression of the Gene in Escherichia coli, and Purification and Characterization of the Gene Product^1
• Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Novel Reactivity of Dibenzothiophene Monooxygenase from Bacillus subtilis WU-S2B
• Dibenzothiophene Desulfurizing Enzymes from Moderately Thermophilic Bacterium Bacillus subtilis WU-S2B : Purification, Characterization and Overexpression(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Gene Cloning and Characterization of Mycobacterium phlei Flavin Reductase Involved in Dibenzothiophene Desulfurization(Enzymology, Protein Engineering, and Enzyme Technology)
• Thermostable Flavin Reductase That Couples with Dibenzothiophene Monooxygenase, from Thermophilic Bacillus sp. DSM411 : Purification, Characterization, and Gene Cloning
• Enhancement and Stabilization of Desulfurization Activity of Rhodococcus erythropolis KA2-5-1 by Feeding Ethanol and Sulfur Components(BIOCHEMICAL ENGINEERING)
• Cloning and Expression of Aspergillus niger icdA Gene Encoding Mitochondrial NADP^+-Specific Isocitrate Dehydrogenase
• Biodesulfurization of Dibenzothiophene and Its Derivatives through the Selective Cleavage of Carbon-Sulfur Bonds by a Moderately Thermophilic Bacterium Bacillus subtilis WU-S2B
• Identification of the Gene Encoding a NAD(P)H-Flavin Oxidoreductase Coupling with Dibenzothiophene (DBT)-Desulfurizing Enzymes from the DBT-Nondesulfurizing Bacterium Paenibacillus polymyxa A-1
• Determination of Nucleotide Sequence Related to the Plasmid Replication Region in Enterococcus faecalis and Its Application to a New Shuttle Vector
• Analysis of Dibenzothiophene Metabolic Pathway in Mycobacterium Strain G3
• Analyses of Substrate Specificity of the Desulfurizing Bacterium Mycobacterium sp. G3
• Gibberellin Biosynthesis in Bambusoideae
• Analysis of Oxidation Sensitivity of Maleate cis-trans Isomerase from Serratia marcescens
• Molecular Analysis of Maleate cis-trans Isomerase from Thermophilic Bacteria
• Replacement Recombination in Coryneform Bacteria: High Efficiency Integration Requirement for Non-methylated Plasmid DNA
• Pyrophosphate-dependent Phosphofructokinase : Its Oligomeric Forms in Mature Green Tomato Fruit
• Mold Pectinase Modified with Dialdehyde Derivatives of Dextran and Cellulose
• Saccharification of Beet Pulp and Its Reduced Derivatives
• Sugar Composition of Beet Pulp Polysaccharides and Their Enzymatic Hydrolysis
• soxRS Gene Increased the Level of Organic Solvent Tolerance in Escherichia coli
• A Close Correlation between Improvement of Organic Solvent Tolerance Levels and Alteration of Resistance toward Low Levels of Multiple Antibiotics in Escherichia coli
• Characterization of an Isoform of Rice Starch Branching Enzyme, RBE4, in Developing Seeds
• Kinetic Analysis of Microbial Desulfurization of Model and Light Gas Oils Containing Multiple Alkyl Dibenzothiophenes
• Improvement of Desulfurization Activity in Rhodococcus erythropolis KA2-5-1 by Genetic Engineering
• Gene Encoding a Dextransucrase-like Protein in Leuconostoc mesenteroides NRRL B-512F
• ENDOGENOUS GIBBERELLIN LEVELS DURING GERMINATION OF BARLEY SEEDS
• Fluctuation of Endogenous Gibberellin and Abscisic Acid Levels in Germinating Seeds of Barley
• Aggregated Form of Dextransucrases from Leuconostoc mesenteroides NRRL B-512F and Its Constitutive Mutant
• Condensation of Dextran-dialdehyde with Amino Acids under Nonreductive Conditions
• Detection of the IAA Biosynthetic Pathway from Tryptophan via Indole-3-Acetamide in Bradyrhizobium spp
• Levels of IAA, Cytokinins ABA and Ethylene in Rice Plants as Affected by a Gibberellin Biosynthesis Inhibitor, Uniconazole-P.
• PCP Award PRESENCE OF A PATHWAY FOR THE BIOSYNTHESIS OF AUXIN VIA INDOLE-3-ACETAMIDE IN TRIFOLIATA ORANGE.
• Presence of a Pathway for the Biosynthesis of Auxin via Indole-3-Acetamide in Trifoliata Orange
• ANALYSIS OF ENDOGENOUS GIBBERELLINS ANDα-AMYLASE ACTIVITY IN GERMINATING RICE SEEDS
• DISTRIBUTION OF GA, IAA AND ABA IN WEEPING AND UPRIGHT TYPES OF JAPANESE CHERRY, Prunus spachiana
• ENDOGENOUS GIBBERELLIN IN NORMAL AND WEEPING CHERRY
• Application of solid-phase extraction to the analysis of the isomers generated in biodesulfurization against methylated dibenzothiophenes
• Isolation of the Pseudomonas aeruginosa Gene Affecting Uptake of Dibenzothiophene in n-Tetradecane
• Isolation of a Recombinant Desulfurizing 4,6-Dipropyl Dibenzothiophene in n-Tetradecane
• Fluctuation and Localization of Endogenous Gibberellins in Rice(Organic Chemistry)
• Purification and Characterization of Dibenzothiophene Sulfone Monooxygenase and FMN-Dependent NADH Oxidoreductase from the Thermophilic Bacterium Paenibacillus sp.Strain A11-2
• Cloning and Expression of the Gene Encoding the Thermophilic NAD(P)H-FMN Oxidoreductase Coupling with the Desulfurization Enzymes from Paenibacillus sp.A11-2
• Characterization and Distribution of IS1164 that Exists in the High Molecular Mass Nitrile Hydratase Gene Cluster of the Industrial Microbe Rhodococcus rhodochrous J1
• The Kobe CK carbonaceous chondrite : petrography, mineralogy and metamorphism
• Production of p-Aminosalicylic Acid through Enzymatic Kolbe-Schmitt Reaction Catalyzed by Reversible Salicylic Acid Decarboxylase
• Improvement of 2'-Hydroxybiphenyl-2-sulfinate Desulfinase, an Enzyme Involved in the Dibenzothiophene Desulfurization Pathway, from Rhodococcus erythropolis KA2-5-1 by Site-Directed Mutagenesis
• Selective Cleavage of the Two C-S Bonds in Asymmetrically Alkylated Dibenzothiophenes by Rhodococcus erythropolis KA2-5-1
• Isolation and Characterization of a Novel Fucoidan-Degrading Microorganism
• Novel Reactivity of Dibenzothiophene Monooxygenase from Bacillus subtilis WU-S2B
• Desulfurization Characteristics of Thermophilic Paenibacillus sp.Strain A11-2 against Asymmetrically Alkylated Dibenzothiophenes
• A Highly Active Producer of Coproporphyrin III and Uroporphyrin III
• Identification of Porphyrins Produced from Isopropanol by Arthrobacter hyalinus

もっと見る 閉じる

スポンサーリンク