Biodesulfurization of Dibenzothiophene and Its Derivatives through the Selective Cleavage of Carbon-Sulfur Bonds by a Moderately Thermophilic Bacterium Bacillus subtilis WU-S2B

スポンサーリンク

概要

• 論文の詳細を見る

• Heterocyclic organosulfur compounds such as dibenzothiophene (DBT) in petroleum cannot be completely removed by hydrodesulfurization using chemical catalysts. A moderately thermophilic bacterium Bacillus subtilis WU-S2B, which could desulfurize DBT at 50℃ through the selective cleavage of carbon-sulfur (C-S) bonds, was newly isolated. At 50℃, growing cells of WU-S2B could degrade 0.54 mM DBT within 120 h to produce 2-hydroxybiphenyl, and the resting cells could also degrade 0.81 mM DBT within 12 h. The DBT-desulfurizing ability of WU-S2B is high over a wide temperature range from 30 to 50℃, and highest at 50℃ for both the growing and resting cells, and this is an extremely advantageous property for the practical biodesulfurization. In addition, WU-S2B could also desulfurize DBT derivatives such as 2, 8-dimethylDBT, 4, 6-dimethylDBT and 3, 4-benzoDBT. Therefore, B.subtilis WU-S2B is considered to have more beneficial properties than other desulfurizing bacteria such as Rhodococcus strains previously reported, particularly from the viewpoint of its capacity for thermophilic desulfurization through the C-S bond cleavage.

• 社団法人日本生物工学会の論文
• 2001-03-25

著者

• Kino Kuniki

  Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University

• Usami Shoji

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• Izumi Yoshikazu

  Dep. Of Biotechnology Tottori Univ.

• KIRIMURA Kohtaro

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• Kino K

  Department Of Applied Chemistry School Of Science And Engineering Waseda University

• Kino Kuniki

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Kino Kuniki

  Department Of Applied Chemistry School Of Science And Engineering Waseda University

• Furuya Toshiki

  Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University

• Ishii Yoshitaka

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• NISHII YASUHIRO

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• Usami S

  Waseda Univ. Tokyo Jpn

• Usami S

  Waseda Univ.

• Usami Shoji

  Dept. Biol. Fac. Sci. Nagoya Univ.

• Nishii Yasuhiro

  Department Of Applied Chemistry School Of Science And Engineering Waseda University

• Kino Kuniki

  Dep. Of Applied Chemistry Fac. Of Sci. And Engineering Waseda Univ. 3-4-1 Ohkubo Shinjuku-ku Tokyo 1

• Ishii Yoshitaka

  Institute For Biomedical Engineering Consolidated Research Institute For Advanced Science And Medica

• Kirimura Kohtaro

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Usami Shoji

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Furuya Toshiki

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Ishii Yoshitaka

  Bio-refining Process Laboratory Advanced Technology And Research Institute Petroleum Energy Center

• Ishii Yoshitaka

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• NISHII YASUHIRO

  Department of Applied Chemistry and Biotechnology, Niihama National College of Technology

関連論文

• Identification and characterization of a novel L-amino acid ligase from Photorhabdus luminescens subsp. laumondii TT01
• ZMVHA-B1, the Gene for Subunit B of Vacuolar H^+-ATPase from the Eelgrass Zostera marina L. Is Able to Replace vma2 in a Yeast Null Mutant(PLANT BIOTECHNOLOGY)
• Identification and characterization of a novel L-amino acid ligase from Photorhabdus luminescens subsp. laumondii TT01(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Intergeneric Hybridization between Aspergillus niger and Trichoderma viride by Protoplast Fusion(Microbiology & Fermentation Industry)
• Improvement in Citric Acid Production by Haploidization of Aspergillus niger Diploid Strains
• Intergeneric Protoplast Fusion between Aspergillus niger and Trichoderma viride(Microbiology & Fermentation Industry)
• Haploid Recombinants Formed as Sectors in the Intraspecific Fusants of Aspergillus niger Producing Citric Acid
• Identification and characterization of a novel L-amino acid ligase from Photorhabdus luminescens subsp. laumondii TT01
• Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Novel Reactivity of Dibenzothiophene Monooxygenase from Bacillus subtilis WU-S2B
• Dibenzothiophene Desulfurizing Enzymes from Moderately Thermophilic Bacterium Bacillus subtilis WU-S2B : Purification, Characterization and Overexpression(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Gene Cloning and Characterization of Mycobacterium phlei Flavin Reductase Involved in Dibenzothiophene Desulfurization(Enzymology, Protein Engineering, and Enzyme Technology)
• Thermostable Flavin Reductase That Couples with Dibenzothiophene Monooxygenase, from Thermophilic Bacillus sp. DSM411 : Purification, Characterization, and Gene Cloning
• Enhancement and Stabilization of Desulfurization Activity of Rhodococcus erythropolis KA2-5-1 by Feeding Ethanol and Sulfur Components(BIOCHEMICAL ENGINEERING)
• Cloning and Expression of Aspergillus niger icdA Gene Encoding Mitochondrial NADP^+-Specific Isocitrate Dehydrogenase
• Biodesulfurization of Dibenzothiophene and Its Derivatives through the Selective Cleavage of Carbon-Sulfur Bonds by a Moderately Thermophilic Bacterium Bacillus subtilis WU-S2B
• Identification of the Gene Encoding a NAD(P)H-Flavin Oxidoreductase Coupling with Dibenzothiophene (DBT)-Desulfurizing Enzymes from the DBT-Nondesulfurizing Bacterium Paenibacillus polymyxa A-1
• Enzymatic Synthesis of l-Menthyl α-Maltoside and l-Menthyl α-Maltooligosides from l-Menthyl α-Glucoside by Cyclodextrin Glucanotransferase
• Continuous Degradation of Dimethyl Sulfoxide to Sulfate Ion by Hyphomicrobium denitrificans WU-K217(ENVIRONMENTAL BIOTECHNOLOGY)
• Enzymatic Synthesis of α-Arbutin by α-Anomer-Selective Glucosylation of Hydroquinone Using Lyophilized Cells of Xanthomonas campestris WU-9701
• α-Anomer-Selective Glucosylation of (+)-Catechin by the Crude Enzyme, Showing Glucosyl Transfer Activity, of Xanthomonas campestris WU-9701
• α-Anomer-Selective Glucosylation of Menthol with High Yield through a Crystal Accumulation Reaction Using Lyophilized Cells of Xanthomonas campestris WU-9701
• Selective and Continuous Degradation of Carbazole Contained in Petroleum Oil by Resting Cells of Sphingomonas sp. CDH-7
• Determination of Nucleotide Sequence Related to the Plasmid Replication Region in Enterococcus faecalis and Its Application to a New Shuttle Vector
• Purification and Characterization of a Novel β-Agarase from an Alkalophilic Bacterium, Alteromonas sp.E-1
• Anomer-Selective Glucosylation of l-Menthol by Yeast α-Glucosidase
• Anomer-Selective Glucosylation of l-Menthol Using Lyophilized Cells of Saccharomyces cerevisiae
• Anomer Selective Formation of l-Menthyl α-D-Glucopyranoside by α-Glucosidase-catalyzed Reaction
• Purification of Extracellular Lipases from Trichosporon fermentans WU-C12
• Lipase Production from Hydrocarbons by Trichosporon fermentans WU-C12 in the Presence of Surfactants
• Lipase Production by Trichosporon fermentans WU-C12, a Newly Isolated Yeasts
• Production of β-Fructofuranosidase Showing Fructose-Transferring Activity by Penicillium frequentans (P. glabrum)
• Ester Synthesis by a Crude Lipase of Rhizopus oligosporus in an Aqueous System
• 1Dp13 Phage engineering II : design for new hostrange of Ralstonia solanacearum phage RSM
• Monitoring of Phytopathogenic Ralstonia solanacearum Cells Using Green Fluorescent Protein-Expressing Plasmid Derived from Bacteriophage φRSS1(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Analysis of nodule specific cysteine protease of Lotus japonicus
• Minichromosome Formation in Chlorella Cells Irradiated with Electron Beams(GENETICS,MOLECULAR BIOLOGY,AND GENE ENGINEERING)
• Digestion of Chlorella Cells by Chlorovirus-encoded Polysaccharide Degrading Enzymes
• Molecular Cloning and Characterization of Two cDNAs Encoding Asparagine Synthetase from Astragalus sinicus Nodules
• Two Catalytic Domains of Chlorella Virus CVK2 Chitinase
• Cloning and alaysis of a nodulin gene which encodes protease of Astragalus sinicus (Renge-sou)
• Contribution of Cyanide-Insensitive Respiratory Pathway, Catalyzed by the Alternative Oxidase, to Citric Acid Production in Aspergillus niger
• Cloning and Sequencing of the Chromosomal DNA and cDNA Encoding the Mitochondrial Citrate Synthase of Aspergillus niger WU-2223L
• Neoagarobiose as a Novel Moisturizer with Whitening Effect
• Retrotransposon-type Reverse Transcriptase in Cblamydomonas reinbardtii
• Intraspecific Protoplast Fusion of Citric Acid-Producing Strains of Aspergillus niger
• Direct Production of Citric Acid from Starch by a 2-Deoxyglucose-Resistant Mutant Strain of Aspergillus niger
• Formation of Autodiploid Strains in Aspergillus niger and Their Application to Citric Acid Production from Starch
• Selection of Mutants of Aspergillus niger Showing Enhanced Productivity of Citric Acid from Starch in Shaking Culture
• Citric Acid Production from Soluble Starch in Shaking Culture by the Selected Mutants of Aspergillus niger
• Effect of Ionic Strength on Optimum pH of Immobilized Trypsin on a Copolymer of 2-Acryloylamino-2-Methylpropanesulfonic Acid and Acrylic Acid
• Cellular Localization of the Constitutive β-Glucosidase in Trichoderma viride
• Effects of the Addition of Laminaran on β-Glucosidase Production by Trichoderma viride
• Regeneration of the mycelial protoplasts of Aspergillus niger
• Plant Homologues of Components of MAPK (Mitogen-Activated Protein Kinase) Signal Pathways in Yeast and Animal Cells : ENVIRONMENTAL AND STRESS RESPONSES : GENE STRUCTURE AND EXPRESSION
• A PLANT PROTEIN KINASE THAT IS ACTIVATEDBY WOUNDING
• Selective α-Glucosylation of Eugenol by α-Glucosyl Transfer Enzyme of Xanthomonas campestris WU-9701(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Interfacial Properties between Aqueous and Organic Phases in AOT Reversed Micellar System for Lysozyme Extraction
• A Novel L-Amino Acid Ligase Is Encoded by a Gene in the Phaseolotoxin Biosynthetic Gene Cluster from Pseudomonas syringae pv. phaseolicola 1448A
• A Novel L-Amino Acid Ligase from Bacillus licheniformis(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Kinetic Analysis of Microbial Desulfurization of Model and Light Gas Oils Containing Multiple Alkyl Dibenzothiophenes
• Improvement of Desulfurization Activity in Rhodococcus erythropolis KA2-5-1 by Genetic Engineering
• Resistance Acquisition of Thiobacillus thiooxidans upon Cadmium and Zinc Ion Addition and Formation of Cadmium Ion-Binding and Zinc Ion-Binding Proteins Exhibiting Metallothionein-Like Properties
• Isolation of Bacteria Degrading Carbazole under Microaerobic Conditions, i.e. Nitrogen Gas Substituted Conditions
• Enzymatic Synthesis of α-Anomer-Selective D-Glucosides Using Maltose Phosphorylase
• Thermostable ATP Regeneration System Using Polyphosphate Kinase from Thermosynechococcus elongatus BP-1 for D-Amino Acid Dipeptide Synthesis(BIOCHEMICAL ENGINEERING)
• Substrate Specificity of Thermostable D-Alanine-D-alanine ligase from Thermotoga maritima ATCC 43589
• D-Amino Acid Dipeptide Production Utilizing D-Alanine-D-Alanine Ligases with Novel Substrate Specificity(Enzymology, Protein Engineering, and Enzyme Technology)
• Expression of Alternative Oxidase Gene (aox1) at the Stage of Single-Cell Conidium in Citric Acid-Producing Aspergillus niger(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Expression Analysis of Alternative Oxidase Gene (aox1) with Enhanced Green Fluorescent Protein as Marker in Citric Acid-Producing Aspergillus niger(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Production of Cellulase and Citric Acid by the Intergeneric Fusants Obtained via Protoplast Fusion between Aspergillus niger and Trichoderma viride(Microbiology & Fermentation Industry)
• Citric Acid Production from Xylan and Xylan Hydrolysate by Semi-Solid Culture of Aspergillus niger
• Isolation of Dimethyl Sulfone-Degrading Microorganisms and Application to Odorless Degradation of Dimethyl Sulfoxide
• Oxidative Degradation of Dimethyl Sulfoxide by Cryptococcus humicolus WU-2, a Newly Isolated Yeast(ENVIRONMENTAL BIOTECHNOLOGY)
• Extraction of Proteins by Reversed Micellar Solution in a Packed Column
• 4'-Galactosyllactose Production in a Jar Fermentor by Cryptococcus laurentii OKN-4
• Biocatalytic Synthesis of Dihydroxynaphthoic Acids by Cytochrome P450 CYP199A2
• The Kobe CK carbonaceous chondrite : petrography, mineralogy and metamorphism
• Enhancement of L-tryptophan 5-hydroxylation activity by structure-based modification of L-phenylalanine 4-hydroxylase from Chromobacterium violaceum
• Enhancement of L-tryptophan 5-hydroxylation activity by structure-based modification of L-phenylalanine 4-hydroxylase from Chromobacterium violaceum(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• A New Method of Synthesis of Alkyl β-Glycosides Using Sucrose as Sugar Donor
• Citric Acid Production by Surface Culture Using Aspergillus niger : Kinetics and Simulation
• A New Method of Synthesis of Alkyl β-Glycosides Using Sucrose as Sugar Donor
• Enzymatic Synthesis of α-Anomer-Selective D-Glucosides Using Maltose Phosphorylase
• Increases in Gene-Targeting Frequencies Due to Disruption of kueA as a ku80 Homolog in Citric Acid-Producing Aspergillus niger
• Production of p-Aminosalicylic Acid through Enzymatic Kolbe-Schmitt Reaction Catalyzed by Reversible Salicylic Acid Decarboxylase
• Improvement of 2'-Hydroxybiphenyl-2-sulfinate Desulfinase, an Enzyme Involved in the Dibenzothiophene Desulfurization Pathway, from Rhodococcus erythropolis KA2-5-1 by Site-Directed Mutagenesis
• Isolation and Characterization of a Novel Fucoidan-Degrading Microorganism
• Novel Reactivity of Dibenzothiophene Monooxygenase from Bacillus subtilis WU-S2B
• High-yield Production of cis, cis-Muconic Acid from Catechol in Aqueous Solution by Biocatalyst
• Biocatalytic Synthesis of Dihydroxynaphthoic Acids by Cytochrome P450 CYP199A2
• Structure-Based Modification of D-Alanine-D-Alanine Ligase from Thermotoga maritima ATCC 43589 for Depsipeptide Synthesis
• A Novel L-Amino Acid Ligase from Bacillus subtilis NBRC3134, a Microorganism Producing Peptide-Antibiotic Rhizocticin
• A Novel L-Amino Acid Ligase Is Encoded by a Gene in the Phaseolotoxin Biosynthetic Gene Cluster from Pseudomonas syringae pv. phaseolicola 1448A
• New L-Amino Acid Ligases Catalyzing Oligopeptide Synthesis from Various Microorganisms
• A Novel L-Amino Acid Ligase from Bacillus subtilis NBRC3134 Catalyzed Oligopeptide Synthesis
• Increases in Gene-Targeting Frequencies Due to Disruption of kueA as a ku80 Homolog in Citric Acid-Producing Aspergillus niger
• Application of protein N-terminal amidase in enzymatic synthesis of dipeptides containing acidic amino acids specifically at the N-terminus(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Biological Reduction of Ferric Iron by Iron- and Sulfur-oxidizing Bacteria
• Application of protein N-terminal amidase in enzymatic synthesis of dipeptides containing acidic amino acids specifically at the N-terminus

もっと見る 閉じる

スポンサーリンク