Resistance Acquisition of Thiobacillus thiooxidans upon Cadmium and Zinc Ion Addition and Formation of Cadmium Ion-Binding and Zinc Ion-Binding Proteins Exhibiting Metallothionein-Like Properties
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概要
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Through subcultivations of Thiobacillus thiooxidans WU-79A in autotrophic media in which the concentrations of Cd^<2+> and Zn^<2+> were increased successively, Cd^<2+>-resistant (CDR) and Zn^<2+>-resistant strains (ZNR) were obtained. The growth of WU-79A was inhibited by the addition of 25 mM Cd^<2+> as well as Zn^<2+>. However, CDR and ZNR could grow without any lag phase in media containing 200 mM Cd^<2+> and 250 mM Zn^<2+>, respectively. CDR and ZNR were able to grow even in media containing up to 400 mM Cd^<2+> and 600 mM Zn^<2+>, respectively, although they exhibited lag phases. CDR could grow in medium containing up to 250 mM Zn^<2+>, as could ZNR in medium containing up to 200 mM Cd^<2+>. Cd^<2+>-binding and Zn^<2+>-binding proteins were isolated from CDR and ZNR, respectively, by gel filtration and ion exchange chromatography. The molecular weights of both proteins were estimated to be approximately 13,000 by gel filtration. The fact that there was no strong absorption at 280 nm of the proteins suggested that they had few aromatic amino acids. Broad absorption bands which are typical of mercaptide (metal thiolate) complexes were detected. The properties of the proteins were spectrophotometrically similar to those of metallothionein.
- 社団法人日本生物工学会の論文
- 1989-04-25
著者
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Usami Shoji
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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KIRIMURA Kohtaro
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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Usami Shoji
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Sakamoto K
Osaka Univ. Osaka Jpn
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Sakamoto Kazuya
Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University
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Sakamoto Kazuya
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Yagasaki Makoto
(present Address)technical Research Laboratory Hofu Plant Kyowa Hakko Kogyo
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Kirimura Kohtaro
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Usami Shoji
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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