Haploid Recombinants Formed as Sectors in the Intraspecific Fusants of Aspergillus niger Producing Citric Acid
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概要
- 論文の詳細を見る
Protoplasts of nutritionally complementary strains of Aspergillus niger producing citric acid were treated in a polyethylene glycol slution, plated onto hypertonic minimal medium and the intraspecific fusants were obtained. When transferred and cultivated on minimal medium, almost all of the fusants continued to grow as heterokaryons. However, some fusant colonies formed sectors of the prototrophic strains (sector strains). Most of these sector strains were haploid recombinants and their properties were genetically stable when subcultivated on both minimal and supplemented media. With respect to morphological features and citric acid productivity, the recombinant strains were like either of the parental strains or an intermediate between both parental strains. The rest of the sector strains that were nonconidiation type seemed to be haploid recombinants, although they stopped growing on the third subcultivations on both the minimal and supplemented media.
- 社団法人日本生物工学会の論文
- 1987-10-25
著者
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Usami Shoji
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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LEE Sung
Department of Internal Medicine, University of Ulsan College of Medicine, Asan Medical Center
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Lee S
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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KIRIMURA Kohtaro
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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KAWABE SEIICHIRO
(Present address)Department of Fundamental Natural Science, Okayama University of Science
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Lee Sung
Department Of Aerospace Engineering Korea Advanced Institute Of Science And Technology
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Usami S
Waseda Univ. Tokyo Jpn
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Usami S
Waseda Univ.
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Usami Shoji
Dept. Biol. Fac. Sci. Nagoya Univ.
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Kirimura Kohtaro
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Kirimura Kohtaro
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Usami Shoji
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Kirimura K
Waseda Univ. Tokyo Jpn
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Kawabe S
Okayama Univ. Science Okayama Jpn
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