Production of β-Fructofuranosidase Showing Fructose-Transferring Activity by Penicillium frequentans (P. glabrum)
スポンサーリンク
概要
- 論文の詳細を見る
Microorganisms producing enzymes showing fructose-transferring activities were screened from various samples such as soils, flowers, vegetables and fruits. One fungus isolated from azalea was found to produce β-fructofuranosidase, which shows fructose-trasferring activity, when cultivated in media containing sucrose as a carbon source. From its growth and morphological characteristics, this strain was identified as Penicillium frequentans (P. glabrum). The strain utilized sucrose and raffinose but not inulin; sucrose was the best carbon source for the production of the enzyme. When cultivated for 4 d in a medium containing 15% (w/v) sucrose under optimal conditions, the fructose-transferring activity reached a maximum of 5.4 U/ml. Under these conditions, 95% of total enzyme activity was detected in the cell-free extract but not in the culture filtrate.
- 社団法人日本生物工学会の論文
- 1991-10-25
著者
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Usami Shoji
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Ishii Takashi
Department Of Pharmacology Kanazawa Medical University
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KIRIMURA Kohtaro
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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Uehara Ken'ichi
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Kino Kuniki
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Usami S
Waseda Univ. Tokyo Jpn
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Usami Shoji
Dept. Biol. Fac. Sci. Nagoya Univ.
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CHEN JIACONG
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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Chen Jiacong
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Kirimura Kohtaro
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Usami Shoji
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Ishii T
Nihon Univ. Tokyo Jpn
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Ishii Takashi
Department Of Neurosurgery Bokuto Municipal Hospital Of Metropolitan Tokyo
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Ishii Takashi
Department Of Animal Science Faculty Of Agriculture Kyoto University
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