Role of Plasma S-Nitrosothiols in Regulation of Blood Pressure in Anesthetized Rabbits with Special References to Hypotensive Effects of Acetylcholine and Nitrovasodilators

スポンサーリンク

概要

• 論文の詳細を見る

• The regulatory role of plasma nitrosothiols (R-SNOs) under steady-state conditions and their possible contribution to pharmacological vasodilation were systematically examined in anesthetized rabbits. Nitrosocystein (Cys-NO), S-nitrosoglutathione (G-SNO), and S-nitrosoalbumin (Alb-SNO) were determined by HPLC-Savilles method with respective sensitivities of 1, 1, and 5 nM. These R-SNOs were not detected under steady-state conditions even in the presence of N-ethylmaleimide, a thiol protective agent used to prevent transnitrosation of R-SNOs. Development of plasma Alb-SNO below 300 nM was observed after intravenous injection (i.v.) of nitric oxide (NO) solution (0.1 to 3 ml/g), NOC7 (an NO releasing agent, above 1 μg/kg), and a low dose of Alb-SNO (10 nmol/kg). However, blood pressure was not significantly reduced by NO solution or Alb-SNO. Intravenous injection of a high dose of Alb-SNO (300 nmol/kg) significantly reduced blood pressure with the appearance not only Alb-SNO in micromolar level in plasma, but also G-SNO in lesser degree. Conversely, the hypotensive effect of Cys-NO (300 nmol/kg, i.v.) and G-SNO (300 nmol/kg, i.v.) accompanied development of Alb-SNO (micromolar level), but not Cys-NO or G-SNO in plasma. R-SNOs were not found in plasma during profound hypotension induced by acetylcholine (10 and 30 μg/kg/min, continuous i.v.), glyceryl trinitrate (100 μg/kg, i.v.), sodium nitroprusside (100 μg/kg, i.v.), and isosorbide dinitrate (300 μg/kg, i.v.). These results indicate that R-SNOs do not play an important role under unstimulated condition. In addition, plasma R-SNOs may not be involved in pharmacological vasodilation where contributions of NO or R-SNOs are suggested.

著者

• Kawada Tomie

  Department of Clinical Pharmacy, Faculty of Pharmacy, Musashino University

• Ishibashi Takaharu

  金沢医科大学 看護学部薬理学

• Ishibashi Takaharu

  Pharmacology School Of Nursing Kanazawa Medical University:department Of Pharmacology School Of Medi

• Ishii Takashi

  Department Of Pharmacology Kanazawa Medical University

• Ishibashi Takaharu

  旭川医科大学 薬理

• MIWA Tomoko

  Department of Pharmacology, School of Medicine, Kanazawa Medical University

• SHINKAWA Ikumi

  Department of Pharmacology, School of Medicine, Kanazawa Medical University

• Yoshida Junko

  Department Of Obstetrics And Gynecology Shinshu University School Of Medicine

• Miwa Tomoko

  Department Of Pharmacology School Of Medicine Kanazawa Medical University

• Shinkawa Ikumi

  Department Of Pharmacology School Of Medicine Kanazawa Medical University

• Nishizawa Naoki

  Department Of Medicinal Chemistry Kyoto Pharmaceutical University

• Ishibashi Takaharu

  Department Of Pharmacology Asahikawa Medical College

• Nishio Matomo

  Department Of Endocrinology Kanazawa Medical University

• KAWADA Tomie

  Department of Clinical Pharmacology, Faculty of Pharmacy, Musashino University

• Nishio Matomo

  Department of Pharmacology, School of Medicine, Kanazawa Medical University

関連論文

• The Kampo Medicines Orengedokuto, Bofutsushosan and Boiogito Have Different Activities to Regulate Gene Expressions in Differentiated Rat White Adipocytes : Comprehensive Analysis of Genetic Profiles(Highlighted paper selected by Editor-in-chief, Pharmaco
• Functional Proteomic Analysis of Experimental Autoimmune Myocarditis-Induced Chronic Heart Failure in the Rat
• Factors Influencing Adherence in Outpatients Using Beclomethasone Dipropionate Inhaler for Bronchial Asthma
• Decrease of Nitric Oxide End-products during Coronary Circulation Reflects Elevated Basal Coronary Artery Tone in Patients with Vasospastic Angina
• Regulation of Endothelial Nitric Oxide Synthase and Endothelin-1 Expression by Fluvastatin in Human Vascular Endothelial Cells
• Ultrasound screening for renal and urinary tract anomalies in healthy infants
• 東京都における学校検尿での尿異常陽性率に対する疫学的検討(30年の推移から)
• Mass screening for early detection of congenital kidney and urinary tract abnormalities in infancy
• Arteriovenous Differences in NO^-_2 Kinetics in Anesthetized Rabbits(Pharmacology)
• Quantifying Nanomolar Levels of Nitrite in Biological Samples by HPLC-Griess Method : Special Reference to Arterio-Venous Difference in vivo
• Effects on 4,4'-Diisothiocyanato-stilbene-2,2'-disulfonic Acid (DIDS) and Chlorpromazine on NO_3^- Transport via Anion Exchanger in Erythrocytes : Inertness of DIDS in Whole Blood
• New Mehtods to Evaluate Endothelial Function : A Search for a Marker of Nitric Oxide (NO) In Vivo : Re-evaluation of NOx in Plasma and Red Blood Cells and a Trial to Detect Nitrosothiols
• Evaluation of NOx in the Cardiovascular System : Relationship to NO-Related Compounds In Vivo
• Autoimmunity in chronic fatigue syndrome in children
• Synthesis of an HIV-1 Protease Analogue by the Chemoselective Ligation Method Using a New Disulfide Type Linker
• A-27. Investigation of Early Stage of Ruptured Intracranial Aneurysm with a Special Reference of Fibrinolytic Activity
• A-92. Vertevral-PICA Aneurysms
• A-2. Severe Head Injury and Intracranial Arterial Spasm
• Lipase Production by Trichosporon fermentans WU-C12, a Newly Isolated Yeasts
• Production of β-Fructofuranosidase Showing Fructose-Transferring Activity by Penicillium frequentans (P. glabrum)
• Ester Synthesis by a Crude Lipase of Rhizopus oligosporus in an Aqueous System
• Forward and Backward Memory Span Assessment in Japanese Children Diagnosed with Attention Deficit and Hyperactivity Disorder, Autistic Disorder, and Asperger's Disorder
• HYPOTENSIVE MECHANISMS OF CARVEDILOL
• A Practical Procedure for Achieving a Steady State of NOx Concentration in Plasma : with Special Reference to the NOx Content of Japanese Daily Food
• Mitochondrial DNA Point Mutation at Nucleotide Pair 3316 in a Japanese Family with Heterogeneous Phenotypes of Diabetes
• Rapidly Progressive Glomerulonephritis Concomitant with Diabetic Nephropathy
• Potentiating Effects of Calcium Chelators on Basal and Stimulated Aldosterone Production by Bovine Adrenal Glomerulosa Cells In Vitro
• Significance of Asymmetric Dimethylarginine (ADMA) Concentrations During Coronary Circulation in Patients With Vasospastic Angina
• Nitrate ion enters into erythrocytes through DIDS-sensitive mechanism
• Significance of asymmetric dimethylarginine (ADMA) in the coronary circulation in patients with vasospastic angina
• Effects of Chronic Cigarette Smoking on Endothelial Function in Healthy Young Men (Smoking 1 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• PALYTOXIN FORMS NEW IONIC CHANNELS
• Different Disappearance Rates of Plasma Nitrite (NO2−) Contribute to Apparent Steady-State Arterio-Venous Differences in Anesthetized Animals
• Changes in the expression of E-cadherin repressors, Snail, Slug, SIP1, and Twist, in the development and progression of ovarian carcinoma : the important role of Snail in ovarian tumorigenesis and progression
• In Vivo and in Vitro Effects of a Protease Inhibitor (E-64) on Cell Division of Xenopus eggs : Biochemistry
• Human amniotic epithelial cells are promising transgene carriers for allogeneic cell transplantation into liver
• The Secretory Cells of the Goat Oviductal Epithelium : An Ultrastructural Study
• The Secretory Cells of the Goat Oviductal Epithelium: An Ultrastructural Study
• Study on feelings of school avoidance, depression, and character tendencies among general junior high and high school students
• Solid Phase Synthesis of a Mercaptoamide Peptide Using New Linkers
• Role of Plasma S-Nitrosothiols in Regulation of Blood Pressure in Anesthetized Rabbits with Special References to Hypotensive Effects of Acetylcholine and Nitrovasodilators
• Diverse Relaxation Responses of Canine Large and Small Conductive Coronary Arteries to Glyceryl Trinitrate and Nitric Oxide on the One Hand and to 8-Bromoguanosine 3':5' Cyclic Monophosphate on the Other
• DIFFERENCE IN THE TIME COURSE BETWEEN INCREASES IN CORONARY FLOW AND IN EFFLUENT ADENOSINE CONCENTRATION DURING ANOXIA IN THE PERFUSED RAT HEART
• EFFECT OF THE CENTRALLY ACTING ANTITUSSIVES ON ASCITES TUMOR CELLS
• Developmental Study on the Vomeronasal Organ in the Rat Fetus
• Effects of chronic cigarette smoking on endothelial function in young men
• Computer Multi-label Analysis of Secretory Cell Localization in the Sheep Pituitary Gland
• Possible Involvement of Hypothalamic Nucleobindin-2 in Hyperphagic Feeding in Tsumura Suzuki Obese Diabetes Mice
• Enhancement of 5-hydroxytryptamine-induced contraction of the guinea pig ileum by a new sulfur compound, tripropylsulfonium bromide.
• Cyclooxygenase 2 and prostaglandin E_2 regulate the attachment of calcium oxalate crystals to renal epithelial cells
• Effects of Cyanamide, an Aldehyde Dehydrogenase Type 2 Inhibitor, on Glyceryl Trinitrate- and Isosorbide Dinitrate-Induced Vasodilation in Rabbit Excised Aorta and in Anesthetized Whole Animal
• Effects of bunitrolol on adrenergic and serotonergic receptors.
• In Vitro Effects of the New Calcium Antagonist Lacidipine.
• Myocardial Na+ during Ischemia and Accumulation of Ca2+ after Reperfusion: A Study with Monesin and Dichlorobenzamil.
• Cytotoxicity of cortisone-resistant lymphocytes from mice treated with a group A streptococcus or freund's complete adjuvant against tumor cells.
• Effect of Pentazocine on the Cytotoxicity of Cortisone-Resistant Lymphocytes from Mouse Thymus.
• Effect of dextromethorphan and dimemorfan on the neutral lipids of tumor cells.
• Cell growth-inhibitory action of SAGP, an antitumor glycoprotein from Streptococcus pyogenes (Su strain).

もっと見る 閉じる

スポンサーリンク