Functional Proteomic Analysis of Experimental Autoimmune Myocarditis-Induced Chronic Heart Failure in the Rat
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概要
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Experimental autoimmune myocarditis (EAM)-induced heart failure in rats is used to study the pathogenesis of heart failure. Based on a proteomic analysis of soluble (S) and membranous (M) fractions extracted from ventricles of rats with a stable chronic form of EAM-induced heart failure, we assessed changes in protein levels and their correlation to heart functions to gain insights into the pathogenesis and to explore new targets for the treatment of heart failure. Proteins were separated by two-dimensional gel electrophoresis and silver stained spots were analyzed. In the S-fraction, 274±3 spots were detected in the normal (N)-group and 273±6 in the heart failure (HF)-group. In the HF-group, 26 of the spots were increased and 15 were decreased in intensity. In the M-fraction, 277±3 spots were detected in the N-group and 277±2 in the HF-group, with 20 spots increased and 10 decreased in intensity. We analyzed relationships between the expression of these proteins and 11 parameters of heart function, and found all the significantly changed spots to correlate with at least one of the parameters. We analyzed 49 spots that correlated with over 9 parameters of heart function using mass spectrometry, and identified 15 as proteins with increased expression including glucose regulated protein (GRP)78, an endoplasmic-stress related protein, and heat shock protein (HSP)90β, a molecular chaperone, and 4 spots as proteins with decreased expression. It is suggested that in the heart failure model, GRP78 and HSP90β play a role in the protection or deterioration of the heart and may be new targets for treatment.
著者
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Sanzen Yoshiki
Department of Medical Technology, School of Health Sciences, Faculty of Medicine, Niigata University
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Ito Minami
Department of Medical Technology, School of Health Sciences, Faculty of Medicine, Niigata University
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Ohta Yoshimi
Department of Medical Technology, School of Health Sciences, Faculty of Medicine, Niigata University
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Yoshida Yutaka
Department of Structural Pathology Institute of Nephrology, Graduate School of Medical and Dental Sc
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Kawada Tomie
Department of Clinical Pharmacy, Faculty of Pharmacy, Musashino University
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Sato Hiroshi
Division of Pharmacy, Niigata University Medical and Dental Hospital
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Yamamoto Tadashi
Department of Structural Pathology Institute of Nephrology, Graduate School of Medical and Dental Sc
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Nakazawa Mikio
Department of Medical Technology, School of Health Sciences, Faculty of Medicine, Niigata University
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Yamamoto Tadashi
Department Of Cardiology Kouseikai Hospital
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Ito Minami
Dep. Of Medical Technol. School Of Health Sci. Fac. Of Medicine Niigata Univ.
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Sanzen Yoshiki
Dep. Of Medical Technol. School Of Health Sci. Fac. Of Medicine Niigata Univ.
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Ohta Yoshimi
Department Of Cardiology Saiseikai Kurihashi Hospital
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Nakazawa Mikio
Departement Of Medical Technology School Of Health Sciences Faculty Of Medicine Niigata University
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Yoshida Yutaka
Departmanets Of Structural Pathology Institute Of Nephrology Graduate School Of Medical And Dental S
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Sato Hiroshi
Division Of Cardiology Tsukazaki Memorial Hospital
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Yoshida Yutaka
Deparlment Of Internal Medicine School Of Medicine Kobe University
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KAWADA Tomie
Department of Clinical Pharmacology, Faculty of Pharmacy, Musashino University
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Ito Minami
Department of Medical Technology, School of Health Science, Faculty of Medicine, Niigata University
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Yamamoto Tadashi
Departmanets of Structural Pathology, Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University
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Sanzen Yoshiki
Department of Medical Technology, School of Health Science, Faculty of Medicine, Niigata University
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