Functional Proteomic Analysis of Experimental Autoimmune Myocarditis-Induced Chronic Heart Failure in the Rat

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概要

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• Experimental autoimmune myocarditis (EAM)-induced heart failure in rats is used to study the pathogenesis of heart failure. Based on a proteomic analysis of soluble (S) and membranous (M) fractions extracted from ventricles of rats with a stable chronic form of EAM-induced heart failure, we assessed changes in protein levels and their correlation to heart functions to gain insights into the pathogenesis and to explore new targets for the treatment of heart failure. Proteins were separated by two-dimensional gel electrophoresis and silver stained spots were analyzed. In the S-fraction, 274±3 spots were detected in the normal (N)-group and 273±6 in the heart failure (HF)-group. In the HF-group, 26 of the spots were increased and 15 were decreased in intensity. In the M-fraction, 277±3 spots were detected in the N-group and 277±2 in the HF-group, with 20 spots increased and 10 decreased in intensity. We analyzed relationships between the expression of these proteins and 11 parameters of heart function, and found all the significantly changed spots to correlate with at least one of the parameters. We analyzed 49 spots that correlated with over 9 parameters of heart function using mass spectrometry, and identified 15 as proteins with increased expression including glucose regulated protein (GRP)78, an endoplasmic-stress related protein, and heat shock protein (HSP)90β, a molecular chaperone, and 4 spots as proteins with decreased expression. It is suggested that in the heart failure model, GRP78 and HSP90β play a role in the protection or deterioration of the heart and may be new targets for treatment.

著者

• Sanzen Yoshiki

  Department of Medical Technology, School of Health Sciences, Faculty of Medicine, Niigata University

• Ito Minami

  Department of Medical Technology, School of Health Sciences, Faculty of Medicine, Niigata University

• Ohta Yoshimi

  Department of Medical Technology, School of Health Sciences, Faculty of Medicine, Niigata University

• Yoshida Yutaka

  Department of Structural Pathology Institute of Nephrology, Graduate School of Medical and Dental Sc

• Kawada Tomie

  Department of Clinical Pharmacy, Faculty of Pharmacy, Musashino University

• Sato Hiroshi

  Division of Pharmacy, Niigata University Medical and Dental Hospital

• Yamamoto Tadashi

  Department of Structural Pathology Institute of Nephrology, Graduate School of Medical and Dental Sc

• Nakazawa Mikio

  Department of Medical Technology, School of Health Sciences, Faculty of Medicine, Niigata University

• Yamamoto Tadashi

  Department Of Cardiology Kouseikai Hospital

• Ito Minami

  Dep. Of Medical Technol. School Of Health Sci. Fac. Of Medicine Niigata Univ.

• Sanzen Yoshiki

  Dep. Of Medical Technol. School Of Health Sci. Fac. Of Medicine Niigata Univ.

• Ohta Yoshimi

  Department Of Cardiology Saiseikai Kurihashi Hospital

• Nakazawa Mikio

  Departement Of Medical Technology School Of Health Sciences Faculty Of Medicine Niigata University

• Yoshida Yutaka

  Departmanets Of Structural Pathology Institute Of Nephrology Graduate School Of Medical And Dental S

• Sato Hiroshi

  Division Of Cardiology Tsukazaki Memorial Hospital

• Yoshida Yutaka

  Deparlment Of Internal Medicine School Of Medicine Kobe University

• KAWADA Tomie

  Department of Clinical Pharmacology, Faculty of Pharmacy, Musashino University

• Ito Minami

  Department of Medical Technology, School of Health Science, Faculty of Medicine, Niigata University

• Yamamoto Tadashi

  Departmanets of Structural Pathology, Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University

• Sanzen Yoshiki

  Department of Medical Technology, School of Health Science, Faculty of Medicine, Niigata University

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