Determination of Nucleotide Sequence Related to the Plasmid Replication Region in Enterococcus faecalis and Its Application to a New Shuttle Vector

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概要

• 論文の詳細を見る

• The 5.1-kb plasmid pAMα1Δ2, a derivative of the 9.6-kb plasmid pAMα1 which is harbored by Enterococcus faecalis ATCC 14508, has a region necessary for replication in E.faecalis. The nucleotide sequence related to the replication region in pAMα1Δ2 was determined and found to contain an open reading frame of 720-bp encoding a replication protein. The sequence showed 54.5 and 48.5% homology to those encoding the RepAs of plasmids pLA103 from Lactobacillus acidophilus and pFA3 from Neisseria gonorrhoeae, respectively. A recombinant 5.8-kb plasmid, pEFX6, which can be used as a shuttle vector between Escherichia coli and some strains of E.faecalis, was constructed by combining the tetracycline resistance gene of pAMα1 and the replication regions of pAMα1Δ2 and pUC18 for E.faecalis and E.coli, respectively. This shuttle vector was successfully used to clone and express the gelatinase gene from E.faecalis subsp.zymogenes IFO 3989 in E.faecalis C57, a strain showing no gelatinase activity.

• 社団法人日本生物工学会の論文
• 1999-05-25

著者

• Usami Shoji

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• Izumi Yoshikazu

  Dep. Of Biotechnology Tottori Univ.

• KIRIMURA Kohtaro

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• Kino K

  Department Of Applied Chemistry School Of Science And Engineering Waseda University

• Ishii Yoshitaka

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• Usami S

  Waseda Univ. Tokyo Jpn

• Usami S

  Waseda Univ.

• Usami Shoji

  Dept. Biol. Fac. Sci. Nagoya Univ.

• KAMIGAKI KIYOTAKE

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• EBIHARA TADASHI

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• KANAYAMA SHINJI

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• Kanayama Shinji

  Department Of Applied Chemistry School Of Science And Engineering Waseda University:(present Address

• Ishii Yoshitaka

  Institute For Biomedical Engineering Consolidated Research Institute For Advanced Science And Medica

• Kirimura Kohtaro

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Usami Shoji

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Ebihara Tadashi

  Department Of Applied Chemistry School Of Science And Engineering Waseda University

• Ishii Yoshitaka

  Bio-refining Process Laboratory Advanced Technology And Research Institute Petroleum Energy Center

• Ishii Yoshitaka

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Kamigaki Kiyotake

  Department Of Applied Chemistry School Of Science And Engineering Waseda University

• Kirimura Kohtro

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

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