Anomer-Selective Glucosylation of l-Menthol by Yeast α-Glucosidase
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概要
- 論文の詳細を見る
l-Menthol was glucosylated by the α-glucosidase (EC 3. 2. 1. 20) of Saccharomyces cerevisiae using maltose as the glucosyl donor. When 50 mg of l-menthol and 1.6 M maltose in 10 mM citrate-phosphate buffer (pH 5.5) were incubated at 45℃, l-menthyl α-D-glucopyranoside (α-MenG) was α-anomer-selectively formed as a product. The specificity of the α-linkage was confirmed by ^<13>C-NMR analysis. In the reaction mixture after 2 h, α-MenG was mainly accumulated in a crystalline form and the concentration of dissolved α-MenG was constant at 1.4 mM. The molar conversion yield of α-MenG produced based on the supplied l-menthol was maximally 30.7% at 48 h of reaction.
- 社団法人日本農芸化学会の論文
- 1998-07-23
著者
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Usami Shoji
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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NAKAGAWA Hiroyuki
Department of Pathology, Kansai Medical University
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SHIOTA Sumiko
Laboratory of Pathogenic Microbiology, School of Pharmacy, Shujitsu University
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KIRIMURA Kohtaro
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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SHIMURA Susumu
Central Laboratory Lotte Co., Ltd.
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Usami S
Waseda Univ. Tokyo Jpn
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Usami S
Waseda Univ.
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Usami Shoji
Dept. Biol. Fac. Sci. Nagoya Univ.
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YOSHIYAMA Masaaki
Central Laboratory, Lotte Co., Ltd
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Nakagawa Hiroyuki
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Yoshiyama Masaaki
Central Laboratory Lotte Co.ltd.
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Kirimura Kohtaro
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Usami Shoji
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Shimura S
Central Laboratory Lotte Co. Ltd.
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Shimura Susumu
Central Laboratory Lotte Co. Ltd.
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Shiota Sumiko
Laboratory Of Pathogenic Microbiology School Of Pharmacy Shujitsu University
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