Selection of Mutants of Aspergillus niger Showing Enhanced Productivity of Citric Acid from Starch in Shaking Culture
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概要
- 論文の詳細を見る
Mutants with enhanced citric acid production from soluble starch were induced from Aspergillus niger WU-2223L. After UV-irradiation of a conidial suspension of strain WU-2223L, mutants were selected on modified starch-methyl red agar plates on the basis of higher amylolytic activity and acid productivity. The 8 mutants selected showed enhanced citric acid production from soluble starch in shaking culture. Among them, a representative mutant strain, 2M-43,produced 48.0 g/l of citric acid from 120 g/l of soluble starch in 9 d of cultivation in shaking culture, whereas strain WU-2223L produced 35.1 g/l. Glucoamylase activities in the culture filtrates of strains 2M-43 and WU-2223L reached maximum levels of 3.62 U/ml and 2.11 U/ml, respectively, both at 3 d of cultivation, and thereafter decreased.
- 社団法人日本生物工学会の論文
- 1993-03-25
著者
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Usami Shoji
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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KIRIMURA Kohtaro
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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Usami S
Waseda Univ. Tokyo Jpn
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Usami Shoji
Dept. Biol. Fac. Sci. Nagoya Univ.
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RUGSASEEL SUGIMA
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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Kirimura Kohtaro
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Kirimura Kohtaro
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Usami Shoji
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Kirimura K
Waseda Univ. Tokyo Jpn
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Rugsaseel Sugima
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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