Selective and Continuous Degradation of Carbazole Contained in Petroleum Oil by Resting Cells of Sphingomonas sp. CDH-7
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概要
- 論文の詳細を見る
Microbial degradation of carbazole (CA), a model of hard-removal heterocyclic nitrogen compounds contained in petroleum oil, was examined using Sphingomonas sp. CDH-7 isolated from a soil sample by screening for CA-as-similating microorganisms. CDH-7 used CA as a sole source of carbon and nitrogen, and metabolized CA to ammonia via anthranilic acid as an intermediate product. When CDH-7 was cultivated in the medium containing CA at the concentration of 500 mg/l (2.99 mM), CA was completely degraded within 50 h. By the reaction with the resting cells of CDH-7,500 mg/l of CA was completely degraded within 4 h, with 1.64 mM of ammonia accumulated in the reaction mixture. When CA was added at the concentration of 100 mg/l (0.599 mM) periodically to the reaction mixture ten times, 925 mg/l (5.54 mM) of CA was degraded within 48 h by the resting cells, and 4.50 mM of ammonia was accumulated in the reaction mixture with a 75.1% molar conversion yield based on total CA added. The resting cells could almost completely degrade CA in a two-liquidphase system which consists of water and organic solvent, even in the presence of 20% (v/v) isooctane, n-hexane, cyclohexane, and kerosene as a model petroleum oil. In the presence of an organic solvent system such as 20% (v/v) pxylene, toluene, and heptanol, however, CA degradation yields decreased.
- 社団法人日本農芸化学会の論文
- 1999-09-23
著者
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Usami Shoji
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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NAKAGAWA Hiroyuki
Department of Pathology, Kansai Medical University
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KIRIMURA Kohtaro
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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Usami S
Waseda Univ. Tokyo Jpn
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Usami S
Waseda Univ.
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Usami Shoji
Dept. Biol. Fac. Sci. Nagoya Univ.
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TSUJI Kenji
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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MATSUDA Kazuya
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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KURANE Ryuichiro
National Institute of Bioscience and Human-Technology (NIBH)
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Matsuda Kazuya
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Nakagawa Hiroyuki
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Kirimura Kohtaro
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Usami Shoji
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Tsuji Kenji
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Kurane R
National Inst. Advanced Industrial Sci. And Technol. Tsukuba Jpn
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Kurane Ryuichiro
National Institute Of Biosciecne And Human Technology Agency Of Industrial Science And Technology Ministry Of International Trade & Industry
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